Scholarlink

<p>A targeted approach to enhance hypericin biosynthesis in <i>Hypericum perforatum</i> hairy root cultures through strategic selection of <i>Rhizobium rhizogenes</i> strains and chitosan elicitation is presented. Among five tested strains, A4 achieved the highest transformation efficiency in root explants (37.77%), while R1000 excelled in leaf and stem tissues, inducing an average of 10.75 roots per stem with a mean length of 6.585&#xa0;cm. Chitosan treatments revealed a concentration-dependent dual effect: 100.0&#xa0;ppm significantly increased biomass accumulation (dry weight), while 200.0&#xa0;ppm promoted root elongation. High-performance liquid chromatography (HPLC) analysis demonstrated that a 72-h exposure to 100.0&#xa0;ppm chitosan led to maximal production of hypericin and pseudohypericin, coinciding with a 100-fold upregulation of the <i>Hyp1</i> biosynthetic gene. In contrast, prolonged exposure to 50.0&#xa0;ppm chitosan over 30 d triggered a 250-fold increase in <i>PLSK2</i> expression, suggesting distinct regulatory pathways for stress response and metabolite synthesis. These findings underscore the novelty of integrating strain-specific transformation with fine-tuned elicitor dosing to optimize metabolite yield. The work establishes <i>Hyp1</i> as a reliable molecular marker for hypericin biosynthesis and provides a scalable framework for pharmaceutical exploitation of <i>H. perforatum</i> root cultures.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chitosan-mediated enhanced hypericin biosynthesis in St. John’s wort (Hypericum perforatum) hairy root cultures

  • 期刊论文

  • 发表日期：

    2026年2月6日

查看全文
  • Arezoo Haghir-Ebrahimabadi,
  • Yousef Hamidoghli,
  • Farzad Banaei-Asl

摘要

A targeted approach to enhance hypericin biosynthesis in Hypericum perforatum hairy root cultures through strategic selection of Rhizobium rhizogenes strains and chitosan elicitation is presented. Among five tested strains, A4 achieved the highest transformation efficiency in root explants (37.77%), while R1000 excelled in leaf and stem tissues, inducing an average of 10.75 roots per stem with a mean length of 6.585 cm. Chitosan treatments revealed a concentration-dependent dual effect: 100.0 ppm significantly increased biomass accumulation (dry weight), while 200.0 ppm promoted root elongation. High-performance liquid chromatography (HPLC) analysis demonstrated that a 72-h exposure to 100.0 ppm chitosan led to maximal production of hypericin and pseudohypericin, coinciding with a 100-fold upregulation of the Hyp1 biosynthetic gene. In contrast, prolonged exposure to 50.0 ppm chitosan over 30 d triggered a 250-fold increase in PLSK2 expression, suggesting distinct regulatory pathways for stress response and metabolite synthesis. These findings underscore the novelty of integrating strain-specific transformation with fine-tuned elicitor dosing to optimize metabolite yield. The work establishes Hyp1 as a reliable molecular marker for hypericin biosynthesis and provides a scalable framework for pharmaceutical exploitation of H. perforatum root cultures.