<p>Cucumber (<i>Cucumis sativus</i> L.) is an important vegetable crop cultivated in both open and protected soil. The technology of doubled haploids (DH) significantly reduces the time required for developing parental forms of heterosis hybrids to 1 to 2&#xa0;yr. Gynogenesis is the most effective method for obtaining DH lines in cucumber, although its efficiency can vary. The objective of this study was to determine the optimal composition of nutrient media for the efficient production of DH cucumber lines. Unpollinated cucumber ovules from three genotypes were used as explants, and three nutrient media with different mineral bases (Murashige and Skoog (MS) medium, cucumber basal medium, and induction medium for Cucurbitaceae) were tested and modified by the addition of 0.04&#xa0;mg L⁻¹ thidiazuron (TDZ) and 10.0&#xa0;mg L⁻¹ silver nitrate (AgNO₃). The effect of various nutrient media compositions, including 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), gibberellic acid (GA₃), and AgNO₃, was studied on the regeneration of induced ovules. The ploidy of regenerant plants was evaluated using flow cytometry, and economically useful traits of the seed progeny were assessed under spring unheated greenhouse conditions. The combination of 0.04&#xa0;mg L⁻¹ TDZ and 10.0&#xa0;mg L⁻¹ AgNO₃ increased the induction rate of cucumber ovules to 54%, including genotypes with low responsiveness. The highest number of plants (17) was obtained from MS medium supplemented with 3.0&#xa0;mg L⁻¹ BAP and 0.05&#xa0;mg L⁻¹ NAA during seedling regeneration. The introduction of 0.1&#xa0;mg L⁻¹ GA₃ accelerated the production of cucumber lines, with seed production taking 15 mo from the introduction of unpollinated ovules. The seed progeny from regenerant plants exhibited high uniformity, early maturity (40 d from sprouting to flowering), and a high degree of parthenocarpy (69%), and was incorporated into the breeding process.</p>