<p>The slipper orchid <i>Paphiopedilum glaucophyllum</i> J.J. Sm. is an endemic species to Java Island, Indonesia, which is currently facing extinction. This research aimed to enhance the growth of <i>P. glaucophyllum</i> with modifications of growth media and growth maintained under various wavelengths using light-emitting diodes (LEDs). Two-year-old plantlets were cultured in Murashige and Skoog (MS) medium supplemented with banana extract (0, 25.0, 50.0, 75.0, and 100.0&#xa0;g L⁻¹). The plantlets were also cultured in MS medium with 5.0&#xa0;mg L⁻¹ kinetin and 1.0&#xa0;mg L⁻¹ IAA as a positive control. The plantlets were exposed to white, red, blue, and red-blue LEDs for 90 d. The new shoots of <i>P. glaucophyllum</i> emerged between 50.6 and 63.0 d after subculturing, with the fastest shoot emergence and the most optimal number of shoots occurring in a combination of 25.0&#xa0;g L⁻¹ banana extract and blue LED, respectively. A combination of 100.0&#xa0;g L⁻¹ banana extract and blue LED light resulted in optimal growth of plantlet height (1.27&#xa0;cm ± 0.07). The positive control showed the highest number of leaves (2.66 ± 0.23) under white LED light, while a significant increase in leaf length (0.79 ± 0.05) was recorded under red LED light. All treatments produced an optimal number of roots under red LED light except for the negative control. Furthermore, the 50.0&#xa0;g L⁻¹ banana extract produced the highest chlorophyll content under red LED light.&#xa0;These findings suggested that further research into the effects of banana extract and light quality on the growth of <i>Paphiopedilum</i> is a promising avenue for species conservation.</p>