Scholarlink

<p>Moso bamboo (<i>Phyllostachys</i><i> pubescens</i> Mazel ex Houzeau de Lehaie) is a major forest crop in China and Asia. While tissue culture protocols for many sympodial bamboos have been established, there are very limited protocols available for monopodial bamboos, such as moso bamboo. In the current study, shoot initiation, shoot proliferation, root initiation, root proliferation, pre-hardening, and acclimatization protocols were established for moso bamboo using axillary buds as explants under a controlled laboratory environment. Among various plant growth regulators used, Murashige and Skoog’s medium supplemented with 9.0&#xa0;µM BAP and 1.0&#xa0;µM meta-topolin showed the highest effectiveness in promoting shoot proliferation, such as maximum number of shoots (81.67 ± 7.38) per explant, shoot length (2.09 ± 0.14), and leaves (68.11 ± 6.65). Healthy shoots were shifted to basal Murashige and Skoog (MS) medium containing rooting hormones IBA, NAA, and IAA at different concentrations. MS medium with 2.5&#xa0;µM IBA induced the highest rooting percentage (66.63%) and formed thick, shiny, golden brown roots, underscoring IBA’s efficacy as a root-inducing hormone. Regenerated rooted plantlets were transferred to jar containing sand enriched with basal liquid MS medium. After 15 to 20 d, well-rooted plantlets were transferred to 1:1:1 (<i>v/v</i>) sand:soil:vermiculite mixture under greenhouse conditions for acclimatization. The genetic consistency of these <i>in vitro</i> regenerated plantlets was verified using PCR-based molecular markers and flow cytometric analysis. All the 11 RAPD markers, 9 SCoT markers, 4 ISSR markers, and 2C nuclear DNA content displayed identical genetic profiles of the tissue culture-raised <i>P. pubescens</i> plantlets. This optimized protocol for direct plant regeneration underscored the safety and reliability of nodal culture for multiplying true-to-type plants to meet the industrial demands.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimization of an efficient micropropagation system from the axillary buds of Phyllostachys pubescens: Chinese moso bamboo

  • 期刊论文

  • 发表日期：

    2025年11月26日

查看全文
  • Anita Kumari,
  • Kiran Devi,
  • Palak Sharma,
  • Ram Kumar Sharma,
  • Rohit Joshi

摘要

Moso bamboo (Phyllostachys pubescens Mazel ex Houzeau de Lehaie) is a major forest crop in China and Asia. While tissue culture protocols for many sympodial bamboos have been established, there are very limited protocols available for monopodial bamboos, such as moso bamboo. In the current study, shoot initiation, shoot proliferation, root initiation, root proliferation, pre-hardening, and acclimatization protocols were established for moso bamboo using axillary buds as explants under a controlled laboratory environment. Among various plant growth regulators used, Murashige and Skoog’s medium supplemented with 9.0 µM BAP and 1.0 µM meta-topolin showed the highest effectiveness in promoting shoot proliferation, such as maximum number of shoots (81.67 ± 7.38) per explant, shoot length (2.09 ± 0.14), and leaves (68.11 ± 6.65). Healthy shoots were shifted to basal Murashige and Skoog (MS) medium containing rooting hormones IBA, NAA, and IAA at different concentrations. MS medium with 2.5 µM IBA induced the highest rooting percentage (66.63%) and formed thick, shiny, golden brown roots, underscoring IBA’s efficacy as a root-inducing hormone. Regenerated rooted plantlets were transferred to jar containing sand enriched with basal liquid MS medium. After 15 to 20 d, well-rooted plantlets were transferred to 1:1:1 (v/v) sand:soil:vermiculite mixture under greenhouse conditions for acclimatization. The genetic consistency of these in vitro regenerated plantlets was verified using PCR-based molecular markers and flow cytometric analysis. All the 11 RAPD markers, 9 SCoT markers, 4 ISSR markers, and 2C nuclear DNA content displayed identical genetic profiles of the tissue culture-raised P. pubescens plantlets. This optimized protocol for direct plant regeneration underscored the safety and reliability of nodal culture for multiplying true-to-type plants to meet the industrial demands.