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<p>Even though micropropagation has been used to produce massive clones of banana, the hardening process used before field transplantation remains highly time consuming. In this study, ten strains of plant growth-promoting bacteria (PGPB) of the genera <i>Pseudomonas</i> and <i>Bacillus</i> were employed individually as microbial inoculants during the hardening period of tissue cultured Nendran variety of banana plantlets under greenhouse conditions. Further, various growth parameters, including the plant height, leaf number, leaf area, root length, root number, and fresh weight, were analyzed for the PGPB-treated plants in comparison with the untreated control. The treated plants were also studied for the modulation of defense-related enzymes, such as phenylalanine ammonia-lyase (PAL) and peroxidase (PO), along with phenolic compounds and, flavonoids and total carbohydrate and chlorophyll contents. The results revealed that bacterial treatments had a statistically significant positive effect on the growth parameters of banana plants compared to the control. Notably, treatment with bacterial strains resulted in the reduction of the hardening period to 8 wk and pronounced growth enhancement was observed after the 12th week of hardening. Moreover, PCA and NMDS analyses indicated that <i>Bacillus</i> sp. BmB9 and <i>Pseudomonas putida</i> exerted the greatest influence on plant growth during hardening. Furthermore, these treatments significantly improved enzyme activity and chlorophyll content. Thus, the findings of this study demonstrated that microbial treatments have a positive influence on the growth metrics and defense responses in the Nendran variety of banana. However, the specific beneficial effect of selected microorganisms on banana was found to be strain specific, which could be due to both the blend of properties it possesses and its ability to interact with the specific variety of banana used. </p>

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Impact of microbial application on the hardening period and defense signaling activation of micropropagated banana var. Nendran

  • 期刊论文

  • 发表日期：

    2025年11月6日

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  • Reni R Krishna,
  • Maria George,
  • Aswani Ravi,
  • Mary Theresa,
  • Riya Alex,
  • Remakanthan Appukuttan,
  • Radhakrishnan Edayileevettil Krishnankutty

摘要

Even though micropropagation has been used to produce massive clones of banana, the hardening process used before field transplantation remains highly time consuming. In this study, ten strains of plant growth-promoting bacteria (PGPB) of the genera Pseudomonas and Bacillus were employed individually as microbial inoculants during the hardening period of tissue cultured Nendran variety of banana plantlets under greenhouse conditions. Further, various growth parameters, including the plant height, leaf number, leaf area, root length, root number, and fresh weight, were analyzed for the PGPB-treated plants in comparison with the untreated control. The treated plants were also studied for the modulation of defense-related enzymes, such as phenylalanine ammonia-lyase (PAL) and peroxidase (PO), along with phenolic compounds and, flavonoids and total carbohydrate and chlorophyll contents. The results revealed that bacterial treatments had a statistically significant positive effect on the growth parameters of banana plants compared to the control. Notably, treatment with bacterial strains resulted in the reduction of the hardening period to 8 wk and pronounced growth enhancement was observed after the 12th week of hardening. Moreover, PCA and NMDS analyses indicated that Bacillus sp. BmB9 and Pseudomonas putida exerted the greatest influence on plant growth during hardening. Furthermore, these treatments significantly improved enzyme activity and chlorophyll content. Thus, the findings of this study demonstrated that microbial treatments have a positive influence on the growth metrics and defense responses in the Nendran variety of banana. However, the specific beneficial effect of selected microorganisms on banana was found to be strain specific, which could be due to both the blend of properties it possesses and its ability to interact with the specific variety of banana used.