<p>Banana (<i>Musa</i> L.) is a staple food in several countries and is distributed worldwide. The USA maintains a collection of banana germplasm at the USDA ARS Tropical Agriculture Research Station in Puerto Rico. Long-term preservation of <i>Musa</i> genetic resources is carried out at the ARS National Laboratory for Genetic Resources Preservation in Colorado. This study tested the effect of six commonly used plant growth regulators (PGRs) (BA, IAA, IBA, NAA, TDZ, and zT) and their concentration in a Murashige and Skoog medium on the growth of corms ≥ 5&#xa0;mm diameter in two accessions of <i>Musa acuminata</i> Colla (TARS 17135 and TARS 17426). The higher the number of cultures with ≥ 5-mm corm diameter, the lower the number of test tubes with this size of corm cultures that must be cultured to achieve a particular number of this size of corms. After three propagation cycles (each 6 wk long), the largest number of corm cultures with ≥ 5-mm diameter was observed in the treatment with 4.5 and 6.5&#xa0;mg L⁻¹ BA; however, other PGRs produced a similar number of this corm size. The study provides information on PGRs supporting growth of a specific corm diameter <i>in vitro</i>. This information is absent in the literature.</p>