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<p>In addressing the limitations of conventional chestnut propagation, this study explored how varying Fe-EDDHA concentrations (0, 5.6, and 11.2&#xa0;mg L<sup>−1</sup>) in different media (Murashige and Skoog, woody plant medium, and Gresshoff and Doy) affected the growth and biochemical traits of the hybrid chestnut variety ‘Marsol’ (<i>C. sativa</i> × <i>C. crenata</i>). Key factors, such as plant height, callus size, chlorophyll content, leaf color parameters, enzymatic activity, and HPLC-determined components, were assessed. The findings revealed that adding 5.6&#xa0;mg L<sup>−1</sup> Fe-EDDHA to the WPM notably improved plant height (3.89&#xa0;cm) and leaf pigmentation, with the <i>L</i> value reaching a minimum of 33.29 and the SPAD value peaking at 10.67. In contrast, the GD medium promoted shoot proliferation (3.81 shoots per explant). The GD medium, when augmented with 5.6&#xa0;mg L<sup>−1</sup> Fe-EDDHA, yielded the most substantial callus diameter (1.71&#xa0;cm width, 0.92&#xa0;cm length), underscoring its efficacy in promoting cellular differentiation and regeneration potential. HPLC analysis revealed that Fe-EDDHA applications across different media affected biochemical profiles. The application of 5.6&#xa0;mg L<sup>−1</sup> Fe-EDDHA in GD medium promoted the proliferation of cells containing high levels of ascorbic acid, gallic acid, and 3,4-hydroxybenzoic acid. The findings provide valuable insights into optimizing culture media to enhance propagation efficiency for important chestnut cultivars or genotypes.</p>

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Effects of different culture media and Fe-EDDHA applications on the in vitro propagation of chestnut and its biochemical profiles

  • 期刊论文

  • 发表日期：

    2025年8月7日

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  • İbrahim Halil Hatipoğlu

摘要

In addressing the limitations of conventional chestnut propagation, this study explored how varying Fe-EDDHA concentrations (0, 5.6, and 11.2 mg L−1) in different media (Murashige and Skoog, woody plant medium, and Gresshoff and Doy) affected the growth and biochemical traits of the hybrid chestnut variety ‘Marsol’ (C. sativa × C. crenata). Key factors, such as plant height, callus size, chlorophyll content, leaf color parameters, enzymatic activity, and HPLC-determined components, were assessed. The findings revealed that adding 5.6 mg L−1 Fe-EDDHA to the WPM notably improved plant height (3.89 cm) and leaf pigmentation, with the L value reaching a minimum of 33.29 and the SPAD value peaking at 10.67. In contrast, the GD medium promoted shoot proliferation (3.81 shoots per explant). The GD medium, when augmented with 5.6 mg L−1 Fe-EDDHA, yielded the most substantial callus diameter (1.71 cm width, 0.92 cm length), underscoring its efficacy in promoting cellular differentiation and regeneration potential. HPLC analysis revealed that Fe-EDDHA applications across different media affected biochemical profiles. The application of 5.6 mg L−1 Fe-EDDHA in GD medium promoted the proliferation of cells containing high levels of ascorbic acid, gallic acid, and 3,4-hydroxybenzoic acid. The findings provide valuable insights into optimizing culture media to enhance propagation efficiency for important chestnut cultivars or genotypes.