Scholarlink

<p><i>Dendrobium officinale,</i> recognized for its medicinal and edible applications, is classified as an endangered species, necessitating tissue culture techniques for its propagation. Nonetheless, hyperhydricity frequently arises during <i>in vitro</i> culture, severely hindering micropropagation commercialization. The exogenous application of potassium silicate has demonstrated effectiveness in alleviating hyperhydricity; however, its underlying mechanism remains poorly understood. In this study, we explored the silicon-regulated recovery mechanism of hyperhydricity by examining physiological and biochemical parameters and conducting transcriptomic sequencing across three groups: Normal, HH, and HH-Si. Compared with the HH plants, the MDA content of HH-Si plants decreased by 19.50%, while the activities of SOD, POD, and CAT increased by 8.93%, 221.00%, and 113.23%, respectively. These results suggest that silicon may primarily restore hyperhydricity by enhancing the activity of antioxidant enzymes. Transcriptome sequencing revealed that silicon recovered the expression levels of genes involved in multiple pathways, including lignin and flavonoid biosynthesis pathways. Additionally, through correlation analysis, we identified a key transcription factor, MYB61, that is closely related to hyperhydricity. This study provides insights into the occurrence of hyperhydricity, and its recovery mediated by silicon. It also offers a theoretical basis for optimizing the tissue culture conditions and commercial production of <i>D. officinale</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptomic analysis reveals the mechanism of silicon-regulated reversion of hyperhydricity in Dendrobium officinale

  • 期刊论文

  • 发表日期：

    2025年4月1日

查看全文
  • Hongli Shang,
  • Xi Huang,
  • Xinyu Zhang,
  • Ziqi Zheng,
  • Quan Yang,
  • Hongyang Gao

摘要

Dendrobium officinale, recognized for its medicinal and edible applications, is classified as an endangered species, necessitating tissue culture techniques for its propagation. Nonetheless, hyperhydricity frequently arises during in vitro culture, severely hindering micropropagation commercialization. The exogenous application of potassium silicate has demonstrated effectiveness in alleviating hyperhydricity; however, its underlying mechanism remains poorly understood. In this study, we explored the silicon-regulated recovery mechanism of hyperhydricity by examining physiological and biochemical parameters and conducting transcriptomic sequencing across three groups: Normal, HH, and HH-Si. Compared with the HH plants, the MDA content of HH-Si plants decreased by 19.50%, while the activities of SOD, POD, and CAT increased by 8.93%, 221.00%, and 113.23%, respectively. These results suggest that silicon may primarily restore hyperhydricity by enhancing the activity of antioxidant enzymes. Transcriptome sequencing revealed that silicon recovered the expression levels of genes involved in multiple pathways, including lignin and flavonoid biosynthesis pathways. Additionally, through correlation analysis, we identified a key transcription factor, MYB61, that is closely related to hyperhydricity. This study provides insights into the occurrence of hyperhydricity, and its recovery mediated by silicon. It also offers a theoretical basis for optimizing the tissue culture conditions and commercial production of D. officinale.