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<p>The formation of adventitious roots impacts the survival rate of <i>Rosa hybrida</i>. The <i>WOX</i> gene family plays a significant role in the formation and development of adventitious roots in various higher plants, but studies on the function of these genes in roses are relatively sparse. Previous studies have found that the <i>RhWOX318</i> gene plays a crucial role in the formation of adventitious roots in<i> Rosa</i> ‘The Fairy’. Therefore, this study investigates the function of <i>RhWOX318</i> in rose adventitious root formation using qRT-PCR and <i>Arabidopsis</i> transformation and other molecular biology techniques. Results indicate that the expression level of <i>RhWOX318</i> is highest in rose roots and increases as adventitious roots form. Subcellular localization revealed that RhWOX318 is primarily located in the nucleus. Compared to the wild type, transgenic <i>RhWOX318</i> overexpression <i>Arabidopsis</i> displayed longer primary root length. Moreover, <i>Arabidopsis</i> overexpressing <i>RhWOX318</i> lines showed longer primary root length, and formed more robust roots under exogenous additions of 6-BA, GA<sub>3</sub>, and NPA compared with the wild type, but high concentration IBA counteracted the effects of overexpressing <i>RhWOX318</i> in plants. Furthermore, <i>RhWOX318</i> can be induced by externally applied dexamethasone (DEX), which affects the formation of differentiated root apical cells. These findings suggest that overexpressing <i>RhWOX318</i> influences root growth and development by regulating the polar transport of growth factors and, in this way, regulating the differentiation of root apical cells, providing a theoretical basis for the formation of rose and <i>Arabidopsis</i> roots.</p>

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Exploring the regulatory role of RhWOX318 in rose and Arabidopsis root development

  • 期刊论文

  • 发表日期：

    2025年4月22日

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  • Shuang Liang,
  • Daidi Che,
  • Lian Duan,
  • Wuhua Zhang,
  • Yuru Mi,
  • Tao Yang,
  • Jinzhu Zhang,
  • Jie Dong

摘要

The formation of adventitious roots impacts the survival rate of Rosa hybrida. The WOX gene family plays a significant role in the formation and development of adventitious roots in various higher plants, but studies on the function of these genes in roses are relatively sparse. Previous studies have found that the RhWOX318 gene plays a crucial role in the formation of adventitious roots in Rosa ‘The Fairy’. Therefore, this study investigates the function of RhWOX318 in rose adventitious root formation using qRT-PCR and Arabidopsis transformation and other molecular biology techniques. Results indicate that the expression level of RhWOX318 is highest in rose roots and increases as adventitious roots form. Subcellular localization revealed that RhWOX318 is primarily located in the nucleus. Compared to the wild type, transgenic RhWOX318 overexpression Arabidopsis displayed longer primary root length. Moreover, Arabidopsis overexpressing RhWOX318 lines showed longer primary root length, and formed more robust roots under exogenous additions of 6-BA, GA3, and NPA compared with the wild type, but high concentration IBA counteracted the effects of overexpressing RhWOX318 in plants. Furthermore, RhWOX318 can be induced by externally applied dexamethasone (DEX), which affects the formation of differentiated root apical cells. These findings suggest that overexpressing RhWOX318 influences root growth and development by regulating the polar transport of growth factors and, in this way, regulating the differentiation of root apical cells, providing a theoretical basis for the formation of rose and Arabidopsis roots.