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<p>This study aimed to optimize viral cleaning and indexing methods in sweet potato genotypes with potential for ethanol production. Two viral cleaning methods (apical meristem cultivation and thermotherapy) and three different PCR variations (conventional PCR, nested PCR, and real-time PCR) were evaluated to detect two sweet potato viruses, <i>Sweet potato mosaic virus</i> (SPMV) and <i>Sweet potato symptomless mastrevirus 1</i> (SPSMV-1), in four sweet potato genotypes (Amanda, Beatriz, Duda and 520). The results revealed that all sweet potato genotypes were contaminated with SPMV and SPSMV-1 viruses. qPCR was the most accurate and reliable diagnostic technique in asymptomatic and symptomatic plants with a detection rate of 100% for plants infected with SPMV compared to 56.25% for PCR and 75% for nested PCR added PCR. However, for SP SMV-1, nested PCR increased detection sensitivity from 62.5% (PCR) to 93.75%, compared to 87.5% for qPCR. Virus-free materials were generated for all genotypes through meristem cultivation and thermotherapy. The cultivation of apical meristems was the most efficient treatment for viral cleaning, displaying an efficiency of 62.5%. On the other hand, the heat treatment, although less efficient (37.5%), was also capable of generating virus-free plants, with the advantage of being a faster alternative for viral cleaning.</p>

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Evaluation of cleaning methods and viral indexing in sweet potato genotypes

  • 期刊论文

  • 发表日期：

    2025年2月10日

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  • Gabriel Duarte de Oliveira,
  • Micaele Rodrigues de Souza,
  • Jaderson Roney Gomes de Oliveira,
  • Kellen Kauanne Pimenta de Oliveira,
  • Márcio Antônio da Silveira,
  • Wagner de Melo Ferreira,
  • Horllys Gomes Barreto,
  • Solange Aparecida Ságio

摘要

This study aimed to optimize viral cleaning and indexing methods in sweet potato genotypes with potential for ethanol production. Two viral cleaning methods (apical meristem cultivation and thermotherapy) and three different PCR variations (conventional PCR, nested PCR, and real-time PCR) were evaluated to detect two sweet potato viruses, Sweet potato mosaic virus (SPMV) and Sweet potato symptomless mastrevirus 1 (SPSMV-1), in four sweet potato genotypes (Amanda, Beatriz, Duda and 520). The results revealed that all sweet potato genotypes were contaminated with SPMV and SPSMV-1 viruses. qPCR was the most accurate and reliable diagnostic technique in asymptomatic and symptomatic plants with a detection rate of 100% for plants infected with SPMV compared to 56.25% for PCR and 75% for nested PCR added PCR. However, for SP SMV-1, nested PCR increased detection sensitivity from 62.5% (PCR) to 93.75%, compared to 87.5% for qPCR. Virus-free materials were generated for all genotypes through meristem cultivation and thermotherapy. The cultivation of apical meristems was the most efficient treatment for viral cleaning, displaying an efficiency of 62.5%. On the other hand, the heat treatment, although less efficient (37.5%), was also capable of generating virus-free plants, with the advantage of being a faster alternative for viral cleaning.