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<p>Fish cell lines are indispensable tools for virology, biotechnology, and toxicology research. This study established a new marine fish cell line, designated EfE, from the eye tissue of the brown-marbled grouper (<i>Epinephelus fuscoguttatus</i>). The EfE cell line has been stably subcultured for over 70 passages in vitro for more than 300&#xa0;days. It proliferated optimally in Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum at 28°C. Species origin was confirmed by molecular analysis of the mitochondrial <i>CO1</i> gene. Chromosome analysis revealed a diploid count of 48, which is consistent with the karyotype of <i>E. fuscoguttatus.</i> The cell line demonstrated high transfection efficiency (25.6%) with a pEGFP-N1 plasmid, indicating its potential for genetic manipulation. In virus susceptibility tests, EfE cells were highly permissive to red-spotted grouper nervous necrosis virus (RGNNV), developing severe cytopathic effects (CPE), including extensive vacuolation, cell rounding, and detachment. Viral replication was further confirmed by semi-quantitative RT-PCR and the observation of virus particles via transmission electron microscopy (TEM). In conclusion, the novel EfE cell line provides a valuable in vitro model for virus isolation, propagation, investigation of pathogenic mechanisms, and genetic studies.</p>

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Establishment and characterization of a continuous cell line from eye of the brown-marbled grouper (Epinephelus fuscoguttatus) for virological studies

  • 期刊论文

  • 发表日期：

    2026年1月16日

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  • Jiqing Zheng,
  • Jinwu Wang,
  • Ting Xue

摘要

Fish cell lines are indispensable tools for virology, biotechnology, and toxicology research. This study established a new marine fish cell line, designated EfE, from the eye tissue of the brown-marbled grouper (Epinephelus fuscoguttatus). The EfE cell line has been stably subcultured for over 70 passages in vitro for more than 300 days. It proliferated optimally in Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum at 28°C. Species origin was confirmed by molecular analysis of the mitochondrial CO1 gene. Chromosome analysis revealed a diploid count of 48, which is consistent with the karyotype of E. fuscoguttatus. The cell line demonstrated high transfection efficiency (25.6%) with a pEGFP-N1 plasmid, indicating its potential for genetic manipulation. In virus susceptibility tests, EfE cells were highly permissive to red-spotted grouper nervous necrosis virus (RGNNV), developing severe cytopathic effects (CPE), including extensive vacuolation, cell rounding, and detachment. Viral replication was further confirmed by semi-quantitative RT-PCR and the observation of virus particles via transmission electron microscopy (TEM). In conclusion, the novel EfE cell line provides a valuable in vitro model for virus isolation, propagation, investigation of pathogenic mechanisms, and genetic studies.