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<p>A new species, <i>Resinomycena melleoguttata</i>, from the Central Highland of Vietnam is described based on morphology, ecology and nrITS evidence. The species is characterized by tiny orange pleurotoid basidiomata covered with small honey-coloured droplets. Phylogeny derived from the nrITS dataset demonstrates that sequences of the new taxon form a well-supported separate clade within <i>Resinomycena</i>. Confocal microscopy proved useful for clearly observing and locating genus-specific complex features such as dendrohyphidia and oleocystidia. A simple protocol is provided to prepare dried fungal samples for confocal microscopy. The method combines staining of hyphal walls in fixed basidiomata with SCRI Renaissance stain 2200, followed by optical clearing with ClearSee solution.</p>

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Confocal laser scanning microscopy with an efficient sample preparation revealing Resinomycena melleoguttata sp. nov. (Mycenaceae, Agaricales, Basidiomycota) from Central Vietnam

  • 期刊论文

  • 发表日期：

    2025年7月7日

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  • Lyudmila B. Kalinina,
  • Kirill N. Demchenko,
  • Alexey S. Kiryushkin,
  • Thi Ha Giang Pham,
  • Katharina Pawlowski

摘要

A new species, Resinomycena melleoguttata, from the Central Highland of Vietnam is described based on morphology, ecology and nrITS evidence. The species is characterized by tiny orange pleurotoid basidiomata covered with small honey-coloured droplets. Phylogeny derived from the nrITS dataset demonstrates that sequences of the new taxon form a well-supported separate clade within Resinomycena. Confocal microscopy proved useful for clearly observing and locating genus-specific complex features such as dendrohyphidia and oleocystidia. A simple protocol is provided to prepare dried fungal samples for confocal microscopy. The method combines staining of hyphal walls in fixed basidiomata with SCRI Renaissance stain 2200, followed by optical clearing with ClearSee solution.