<p>This work aims to develop a colloidal gold immunochromatographic test strip for the on-site rapid detection of potato leaf roll virus (PLRV). Colloidal gold solution was prepared via the trisodium citrate reduction method. Utilizing the specific binding property between antibodies and gold atoms, rabbit-derived polyclonal antibodies against PLRV were labeled with gold. For the test strip construction, the colloidal gold-labeled anti-PLRV rabbit polyclonal antibodies were sprayed onto glass fiber as the detection antibodies; meanwhile, goat anti-rabbit IgG (immunoglobulin G) at the control line (C line) and anti-PLRV antibodies at the test line (T line) on the nitrocellulose (NC) membrane served as the capture antibodies. A positive result could be visually judged with the naked eye within 5&#xa0;min by the appearance of a red T line. The test strip maintained detectable capability for samples diluted at 2 × 10² (w/v), and exhibited no cross-reactivity with samples infected with five other common potato viruses (PVX, PVY, PVS, PVM, and PVA). Field detection results of potato leaf samples using this test strip were consistent with those obtained by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). This test strip features convenience, simplicity, and rapidity for on-site PLRV detection, requiring no additional equipment. It is particularly suitable for potato planting fields and units with limited testing conditions lacking professional instruments, thus demonstrating broad applicability and strong practicability.</p>