<p>Determining mechanical properties such as the modulus of elasticity and Poisson’s ratio of agricultural products is a crucial initial step in designing optimal tools and machinery systems for mechanised harvesting and postharvest operations. However, this process presents measurement difficulties, particularly in calculating Poisson’s ratio due to limitations in experimental approaches and the organic structure of some agricultural products. This study describes a finite element analysis (FEA) approach for estimating Poisson’s ratio in potato flesh, a solid-like agricultural product sample. Analytical, experimental, and numerical methods were employed in the study. The results clearly demonstrate the success of the FEA approach compared to empirical calculations for determining Poisson’s ratio (relative difference of 7.93%). This study contributes to advancing the understanding of complex deformation phenomena in solid-like agricultural products. Moreover, the application of the FEA approach has significant industrial and research implications for enhancing the critical mechanical properties of agricultural products.</p>