The application of the standard quasi-steady-state approximation to the Michaelis-Menten reaction mechanism is a textbook example of biochemical model reduction, derived using singular perturbation theory. However, determining the specific biochemical conditions that dictate the validity of the standard quasi-steady-state approximation remains a challenging endeavor. Emerging research suggests that the accuracy of the standard quasi-steady-state approximation improves as the ratio of the initial enzyme concentration, \(e_0\) , to the Michaelis constant, \(K_M\) , decreases. In this work, we examine this ratio and its implications for the accuracy and validity of the standard quasi-steady-state approximation as compared to other quasi-steady-state reductions in its proximity. Using standard tools from the analysis of ordinary differential equations, we show that while \(e_0/K_M\) provides an indication of the standard quasi-steady-state approximation’s asymptotic accuracy, the standard quasi-steady-state approximation’s predominance relies on a small ratio of \(e_0\) to the Van Slyke-Cullen constant, K. Here, we define the predominance of a quasi-steady-state reduction when it offers the highest approximation accuracy among other well-known reductions with overlapping validity conditions. We conclude that the magnitude of \(e_0/K\) offers the most accurate measure of the validity of the standard quasi-steady-state approximation.
