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<p>Heat shock protein 90 (HSP90) is a chaperone protein that assists in folding various client proteins and is expressed abundantly in cancer cells, making it a key target for drug discovery and a significant biomarker. In particular, detecting HSP90 secreted into the bloodstream remains challenging in clinical diagnostics. Currently, simple and rapid HSP90 sensors suitable for clinical use are limited, highlighting the need for HSP90 detection technologies designed for point-of-care testing (POCT). In this study, we developed an HSP90 sensor based on a localized surface plasmon resonance (LSPR) platform, functionalized with HSP90-binding peptides via linker molecules. The sensor achieved a detection limit of 0.75&#xa0;µg/mL for HSP90 and could detect HSP90 in 50% serum. Additionally, we found that the length of the linker molecules affects sensor sensitivity, underscoring the importance of optimized molecular design. These results suggest that the LSPR sensor, which enables rapid HSP90 detection with simple equipment, holds promise for cancer diagnosis and therapeutic monitoring in POCT applications.</p>

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Peptide-Functionalized Localized Surface Plasmon Resonance (LSPR) Sensor for Label-Free Detection of HSP90

  • 期刊论文

  • 发表日期：

    2025年11月13日

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  • Kazuto Mochizuki,
  • Yuki Takimoto,
  • Tomoe Nakagawa,
  • Tomoko Gessei

摘要

Heat shock protein 90 (HSP90) is a chaperone protein that assists in folding various client proteins and is expressed abundantly in cancer cells, making it a key target for drug discovery and a significant biomarker. In particular, detecting HSP90 secreted into the bloodstream remains challenging in clinical diagnostics. Currently, simple and rapid HSP90 sensors suitable for clinical use are limited, highlighting the need for HSP90 detection technologies designed for point-of-care testing (POCT). In this study, we developed an HSP90 sensor based on a localized surface plasmon resonance (LSPR) platform, functionalized with HSP90-binding peptides via linker molecules. The sensor achieved a detection limit of 0.75 µg/mL for HSP90 and could detect HSP90 in 50% serum. Additionally, we found that the length of the linker molecules affects sensor sensitivity, underscoring the importance of optimized molecular design. These results suggest that the LSPR sensor, which enables rapid HSP90 detection with simple equipment, holds promise for cancer diagnosis and therapeutic monitoring in POCT applications.