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<p>This study presents an eco-friendly approach to the synthesis and characterization of silver nanoparticles (AgNPs) utilizing a bacterial colicin extract as a reducing agent. Silver nanoparticles were successfully synthesized through the bioreduction of silver nitrate (AgNO₃) using the bioactive components of colicin under mild, non-toxic conditions. The resulting nanoparticles were characterized by UV–Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM). TEM showed that AgNPs are spherical nanoparticles with a range of 5–25 nm. The biosynthesized AgNPs exhibited notable antimicrobial activity against selected pathogenic microorganisms. In addition, AgNPs exhibit anticancer activity against lung cancer cell line A549. Furthermore, molecular docking and molecular dynamics (MD) simulations were employed to investigate the interactions between AgNPs and specific bacterial receptors <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, and <i>Pseudomonas aeruginosa</i>. Docking studies revealed significant binding affinities between AgNPs and the active sites of these receptors, with the highest interaction observed for <i>P. aeruginosa</i>. MD simulations confirmed the stability and favorable energetic profiles of the AgNP–receptor complexes, supporting their promising antibacterial potential. Overall, this work demonstrates the synergistic value of green nanotechnology and computational tools in advancing antimicrobial strategies.</p>

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Insights into the Antibacterial and Anticancer Activity of Novel Silver Nanoparticles Mediated Bacterial Colicin: Sustainable Nanotechnology, In silico and Dynamic Study

  • 期刊论文

  • 发表日期：

    2025年7月9日

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  • Ibrahim M. Abbas,
  • Saba A. Mahdi,
  • Majid S. Jabir

摘要

This study presents an eco-friendly approach to the synthesis and characterization of silver nanoparticles (AgNPs) utilizing a bacterial colicin extract as a reducing agent. Silver nanoparticles were successfully synthesized through the bioreduction of silver nitrate (AgNO₃) using the bioactive components of colicin under mild, non-toxic conditions. The resulting nanoparticles were characterized by UV–Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM). TEM showed that AgNPs are spherical nanoparticles with a range of 5–25 nm. The biosynthesized AgNPs exhibited notable antimicrobial activity against selected pathogenic microorganisms. In addition, AgNPs exhibit anticancer activity against lung cancer cell line A549. Furthermore, molecular docking and molecular dynamics (MD) simulations were employed to investigate the interactions between AgNPs and specific bacterial receptors Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Docking studies revealed significant binding affinities between AgNPs and the active sites of these receptors, with the highest interaction observed for P. aeruginosa. MD simulations confirmed the stability and favorable energetic profiles of the AgNP–receptor complexes, supporting their promising antibacterial potential. Overall, this work demonstrates the synergistic value of green nanotechnology and computational tools in advancing antimicrobial strategies.