<p>Two-dimensional electron system (2DES) has been widely recognized as critical for fundamental physical research and holds significant potential for developing information devices. A 2DES floating on the surface of liquid helium exhibits exceptionally high mobility, tunability, and controllability, making it an ideal candidate for quantum device applications. Reliable electron transfer that preserves electrons’ quantum state is an essential requirement for device fabrication. In this study, we investigate electron transfer for 2DES floating on helium triggered by surface acoustic waves (SAW). By coupling electrons with the evanescent electric field of SAW, we successfully observed acoustoelectric currents. Dynamics of electron transfer were captured in real-time via time-of-flight experiments, allowing us to determine their transfer speed, magnitude, and resistance. By tuning the power of SAW pulses, we achieved a transfer rate from 8.87×10³ to 4.63×10⁵ electrons per pulse, accounting for a fraction between 9.48×10⁻⁶ and 4.95×10⁻⁴ of the entire 2DES. Our study provides a promising approach for fabricating quantum devices based on 2DES floating on helium.</p>