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<p>Butyrate, an important short-chain fatty acid, plays multiple biological roles in animal growth and development. Our previous studies have revealed that <i>SYISL</i> knockout in mother mice can enhance embryonic muscle development in their offspring by influencing the maternal gut microbiota and its metabolite, butyric acid. However, it remains unclear whether butyrate supplementation in sows can enhance the total muscle fiber number in piglets. In this study, we confirmed that supplementing gestating sow diets with 0.05% sodium butyrate significantly increased birth weight and total muscle fiber number in the longissimus dorsi muscle of their offspring. Further studies revealed that maternal butyrate may influence offspring embryonic myogenesis through remodeling maternal gut microbiota composition and enhancing offspring intestinal barrier function. Metabolomic and transcriptomic analyses in the longissimus dorsi muscle revealed that maternal butyrate supplementation in sows modulates arginine and proline metabolism in skeletal muscles of offspring. Integrated analyses of transcriptomics, H3K27ac Cut&amp;Tag in pig muscle, and <i>in vitro</i> experiments demonstrated that maternal butyrate increases HDAC-mediated H3K27ac levels in the longissimus dorsi muscle of piglets and promotes the expression of myogenic genes by enhancing H3K27ac enrichment in their regulatory regions, such as <i>MyoG, Myh7</i>, and <i>Acta1</i>. These results uncover a new mechanism through which butyrate promotes the development of embryonic muscle and support a theoretical foundation for increasing the total muscle fiber number in piglets.</p>

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Butyrate supplementation in sows promotes offspring embryonic muscle development by upregulating HDAC-mediated H3K27ac levels and myogenic gene expression

  • 期刊论文

  • 发表日期：

    2026年2月9日

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  • Hao Zuo,
  • Chen Li,
  • Xiaoyu Niu,
  • Zhibo Ma,
  • Zhifeng Wu,
  • Zeyang Gao,
  • Liqian Sun,
  • Yiming Jia,
  • Mingfei Zhu,
  • Litong Wang,
  • Rong Zheng,
  • Bo Zuo

摘要

Butyrate, an important short-chain fatty acid, plays multiple biological roles in animal growth and development. Our previous studies have revealed that SYISL knockout in mother mice can enhance embryonic muscle development in their offspring by influencing the maternal gut microbiota and its metabolite, butyric acid. However, it remains unclear whether butyrate supplementation in sows can enhance the total muscle fiber number in piglets. In this study, we confirmed that supplementing gestating sow diets with 0.05% sodium butyrate significantly increased birth weight and total muscle fiber number in the longissimus dorsi muscle of their offspring. Further studies revealed that maternal butyrate may influence offspring embryonic myogenesis through remodeling maternal gut microbiota composition and enhancing offspring intestinal barrier function. Metabolomic and transcriptomic analyses in the longissimus dorsi muscle revealed that maternal butyrate supplementation in sows modulates arginine and proline metabolism in skeletal muscles of offspring. Integrated analyses of transcriptomics, H3K27ac Cut&Tag in pig muscle, and in vitro experiments demonstrated that maternal butyrate increases HDAC-mediated H3K27ac levels in the longissimus dorsi muscle of piglets and promotes the expression of myogenic genes by enhancing H3K27ac enrichment in their regulatory regions, such as MyoG, Myh7, and Acta1. These results uncover a new mechanism through which butyrate promotes the development of embryonic muscle and support a theoretical foundation for increasing the total muscle fiber number in piglets.