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<p>Thermo-sensitive genic male-sterile (TGMS) lines are fertile at lower temperatures but male sterile above a critical sterility-inducing temperature (CSIT), which varies substantially across TGMS lines. Such lines are valuable resources for breeding two-line hybrid rice (<i>Oryza sativa</i>). However, as their utility is restricted by the CSIT, understanding its regulatory mechanisms has crucial breeding-associated implications. The <i>thermo-sensitive genic male sterility 5</i> (<i>tms5</i>) mutation is common to most TGMS lines. We previously revealed that <i>tms5</i> mutants failed to recycle cyclic phosphate (cP)-containing ΔCCA-tRNAs, particularly in pollen mother cells, resulting in fewer mature tRNAs, thereby causing TGMS. However, the CSIT-regulatory network in the <i>tms5</i> lines remains unelucidated. Here, we characterized the CSIT-related <i>ribosome-associated quality control 1</i> (<i>OsRqc1</i>) gene. OsRqc1 binds to the 60S ribosomal subunits and functions in ribosome-associated quality control. It also regulates the CSIT in <i>tms5</i> lines by assisting OsVms1 in recruiting these subunits to generate cP-ΔCCA-tRNAs. Variations in a 6-bp repeat in the 5′ untranslated region of <i>OsRqc1</i> in rice subspecies affect its transcription, thereby altering the CSIT in <i>tms5</i> lines. These findings reveal the role of an OsRqc1-OsVms1 module in regulating CSIT, providing valuable insights into genetic variations in <i>OsRqc1</i>. Such results may facilitate the molecular breeding of rice two-line hybrid <i>tms5</i> lines with specific CSITs, which can adapt to regions with different environments.</p>

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The OsRqc1-OsVms1 module regulates the temperature threshold in thermo-sensitive genic male-sterile rice lines

  • 期刊论文

  • 发表日期：

    2025年7月25日

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  • Can Zhou,
  • Bin Yan,
  • Chunyan Liu,
  • Jing Sun,
  • Yuanzhu Yang,
  • Xiaofeng Cao

摘要

Thermo-sensitive genic male-sterile (TGMS) lines are fertile at lower temperatures but male sterile above a critical sterility-inducing temperature (CSIT), which varies substantially across TGMS lines. Such lines are valuable resources for breeding two-line hybrid rice (Oryza sativa). However, as their utility is restricted by the CSIT, understanding its regulatory mechanisms has crucial breeding-associated implications. The thermo-sensitive genic male sterility 5 (tms5) mutation is common to most TGMS lines. We previously revealed that tms5 mutants failed to recycle cyclic phosphate (cP)-containing ΔCCA-tRNAs, particularly in pollen mother cells, resulting in fewer mature tRNAs, thereby causing TGMS. However, the CSIT-regulatory network in the tms5 lines remains unelucidated. Here, we characterized the CSIT-related ribosome-associated quality control 1 (OsRqc1) gene. OsRqc1 binds to the 60S ribosomal subunits and functions in ribosome-associated quality control. It also regulates the CSIT in tms5 lines by assisting OsVms1 in recruiting these subunits to generate cP-ΔCCA-tRNAs. Variations in a 6-bp repeat in the 5′ untranslated region of OsRqc1 in rice subspecies affect its transcription, thereby altering the CSIT in tms5 lines. These findings reveal the role of an OsRqc1-OsVms1 module in regulating CSIT, providing valuable insights into genetic variations in OsRqc1. Such results may facilitate the molecular breeding of rice two-line hybrid tms5 lines with specific CSITs, which can adapt to regions with different environments.