Scholarlink

<p>Whole-genome duplication (WGD) events are widespread across eukaryotes and have played a significant role in moulding the genetic architectures of diverse organisms. In the present study, the newly sequenced genome of a giant ciliated protist, <i>Stentor roeselii</i>, provides an opportunity for the analysis of the collinearity and retention of reciprocal best-hit genes between two <i>Stentor</i> species. As a main result, we have unveiled a previously undetected ancient WGD event shaping the genome of its congener, <i>Stentor coeruleus</i>, a model protist used in cytological and evolutionary studies. Genomes of two congeners, <i>S. coeruleus</i> and <i>S. roeselii</i>, are compared and analyzed, revealing that: (i) the former exhibits a much higher retention rate of colinear-gene pairs (28%) than does <i>S. roeselii</i>, and in <i>S. coeruleus</i>, 75% of genes that have a RBH hit in <i>S. roeselii</i>, have paralogs with high amino-acid identity, consistent with a WGD event in the lineage leading to <i>S. coeruleus</i>; (ii) the <i>S. roeselii</i> genome possesses extremely short intergenic regions, implying that the lengths of intergenic regions are under strong selection; (iii) the unique characteristics of introns may have been shaped in the common ancestor of heterotrichs; (iv) gene families that play a role in activities of multiple protein kinases and voltage-gated ion channels expanded rapidly in the ancestor of both taxa, possibly relating to the remarkable regenerative ability in <i>Stentor</i>. This study offers new insights into the evolutionary dynamics of ciliate genomes, with implications for understanding of the processes underlying the evolution of genomic complexity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Unveiling an ancient whole-genome duplication event in Stentor, the model unicellular eukaryotes

  • 期刊论文

  • 发表日期：

    2025年1月8日

查看全文
  • Weibo Zheng,
  • Chao Li,
  • Zhaorui Zhou,
  • Xiao Chen,
  • Michael Lynch,
  • Ying Yan

摘要

Whole-genome duplication (WGD) events are widespread across eukaryotes and have played a significant role in moulding the genetic architectures of diverse organisms. In the present study, the newly sequenced genome of a giant ciliated protist, Stentor roeselii, provides an opportunity for the analysis of the collinearity and retention of reciprocal best-hit genes between two Stentor species. As a main result, we have unveiled a previously undetected ancient WGD event shaping the genome of its congener, Stentor coeruleus, a model protist used in cytological and evolutionary studies. Genomes of two congeners, S. coeruleus and S. roeselii, are compared and analyzed, revealing that: (i) the former exhibits a much higher retention rate of colinear-gene pairs (28%) than does S. roeselii, and in S. coeruleus, 75% of genes that have a RBH hit in S. roeselii, have paralogs with high amino-acid identity, consistent with a WGD event in the lineage leading to S. coeruleus; (ii) the S. roeselii genome possesses extremely short intergenic regions, implying that the lengths of intergenic regions are under strong selection; (iii) the unique characteristics of introns may have been shaped in the common ancestor of heterotrichs; (iv) gene families that play a role in activities of multiple protein kinases and voltage-gated ion channels expanded rapidly in the ancestor of both taxa, possibly relating to the remarkable regenerative ability in Stentor. This study offers new insights into the evolutionary dynamics of ciliate genomes, with implications for understanding of the processes underlying the evolution of genomic complexity.