<p>Organic room-temperature phosphorescence (RTP) with ultralong lifetime for afterglow has garnered great research interest in the recent decade and a variety of organic afterglow materials have been realized in solid states of crystal/powder, plastics, elastomer, and gel. However, there is limited success in achieving afterglow fluids because of the fast non-radiative relaxations through vibration and collision of phosphors in the flowable state. Here, we report a general strategy to develop afterglow fluids by constructing strong ionic/hydrogen bond networks within ionic polymer and copolymerized ionic phosphors, enabling intense repulsive interactions between the isolated phosphors and matrix in a rigid but flowable environment. Therefore, the aqueous RTP fluid exhibits a lifetime of up to 0.38 s under ambient conditions with tunable persistent luminescence upon excitation of different wavelengths. After removing the water, the dense ionic bond network in the solid state is further enhanced, leading to the improved afterglow performance with a lifetime up to 1.20 s and impressive adhesive properties with an adhesion strength up to 770 kPa. Notably, the RTP fluid exhibits good adhesiveness toward a wide range of substrates such as wood, ceramic, glass, paper, plastics, and metal. With the extraordinary afterglow in the water-sensitive smart adhesive fluids, advanced applications in porcelain restoration, traceable smart adhesion, and flexible afterglow fiber were successfully realized. This study illustrates an effective approach to achieving afterglow in fluid systems, setting a remarkable benchmark for the design of innovative afterglow materials and inspiring future advancements in optoelectronic fluids for interdisciplinary applications.</p>