<p>Nonreciprocal chirality is essential for achieving nonreciprocal circularly polarized luminescence (CPL) emission, which shows great promise in a variety of potential applications due to its convenient handedness inversion just by flipping the sample. Unfortunately, research on nonreciprocal CPL materials still remains severely limited, and further investigations in this direction are urgently needed. Based on our preceding studies dealing with nonreciprocal CPL materials, we in this study synthesized polyurethanes (PU) exhibiting nonreciprocal chirality, originating from oriented hard segments in the hard domains induced by micro-phase separation. By further introducing achiral helical polyacetylene, we observed a red-shift in the apparent Cotton effect, demonstrating the successful transfer of nonreciprocal chirality from PU to the achiral polyacetylene. Further incorporating achiral fluorescent sources into the PU systems containing polyacetylene realized nonreciprocal CPL emission. Our research offers a convenient and universal approach for generating and modulating nonreciprocal chirality and further enabling the realization of nonreciprocal CPL emission derived from polymeric materials.</p>