<p>Inspired by nature, biomimetic photoregulated ion transporters have attracted great interest because of the spatial and temporal control over ion transport, in which photocontrollable anion transport remains a very challenging task. Herein, we demonstrate an anionophore AZC for photoregulating anion transport with a high selectivity for chloride over hydroxide across the lipid membranes. Our strategy is based on a well-designed bidentate iodo-triazole anion receptor equipped with an azobenzene moiety, which recognizes Cl⁻ by C–I halogen bonding and C–H hydrogen bonding and switches its states in the lipid membranes from inactive aggregates to active single molecules through azobenzene photoisomerization, thereby achieving controllable Cl⁻ transport. Compared with <i>trans</i>-AZC, <i>cis</i>-AZC exhibits much higher ion transport activity with an EC₅₀ (the effective concentration required for 50% activity) value at 28 nM, which is one of the highest reported activities to date for halogen bonding anionophores. Finally, it allows the modulation of Cl⁻ transport upon reversible photoisomerization of AZC using alternative ultraviolet and visible light irradiation. This work provides a new strategy for designing stimuli-responsive anion transporters and paves the way for potential applications in remotely controlled membrane transport and treatment of related diseases.</p>