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<p>A comprehensive phytochemical investigation of <i>Centella asiatica</i> leaves collected in Thailand (sample CAT001) led to the isolation and structural elucidation of eighteen compounds, including two novel polyacetylene glucosides, centellatriolosides A and B. Among the identified constituents, three major triterpenoid glycosides—terminoloside, madecassoside, and asiaticoside—were prominently featured. Terminoloside, newly reported in Thai <i>C. asiatica</i>, was consistently observed in substantial amounts across additional regional samples (CAT002, CAT003, and CAT004), suggesting its broad geographical distribution. Structural elucidation was achieved through detailed spectroscopic analysis, including 1D and 2D NMR techniques. Additionally, a conventional HPLC-UV method employing step-gradient elution was evaluated for the simultaneous separation of six structurally related triterpenes—three glycosides and their corresponding aglycones (terminolic, madecassic, and asiatic acids)—which serve as key quality control markers. Although the method enhanced chromatographic resolution, complete separation of isomeric pairs remained challenging, highlighting the need for advanced analytical approaches in <i>C. asiatica</i> quality assessment.</p> Graphical abstract <p></p>

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Phytochemical profiling of Thai Centella asiatica: identification of new polyacetylene glucosides and analytical considerations in HPLC-UV analysis of triterpenoids

  • 期刊论文

  • 发表日期：

    2026年1月20日

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  • Jedsada Maliwong,
  • Phonchanok Reuk-ngam,
  • Nitirat Chimnoi,
  • Pathomthat Srisuk,
  • Supanna Techasakul,
  • Somsak Ruchirawat,
  • Tripetch Kanchanapoom

摘要

A comprehensive phytochemical investigation of Centella asiatica leaves collected in Thailand (sample CAT001) led to the isolation and structural elucidation of eighteen compounds, including two novel polyacetylene glucosides, centellatriolosides A and B. Among the identified constituents, three major triterpenoid glycosides—terminoloside, madecassoside, and asiaticoside—were prominently featured. Terminoloside, newly reported in Thai C. asiatica, was consistently observed in substantial amounts across additional regional samples (CAT002, CAT003, and CAT004), suggesting its broad geographical distribution. Structural elucidation was achieved through detailed spectroscopic analysis, including 1D and 2D NMR techniques. Additionally, a conventional HPLC-UV method employing step-gradient elution was evaluated for the simultaneous separation of six structurally related triterpenes—three glycosides and their corresponding aglycones (terminolic, madecassic, and asiatic acids)—which serve as key quality control markers. Although the method enhanced chromatographic resolution, complete separation of isomeric pairs remained challenging, highlighting the need for advanced analytical approaches in C. asiatica quality assessment.

Graphical abstract