Scholarlink

<p>A saponin fraction from <i>Allium chinense</i> G. Don. bulbs has previously been reported to exhibit cytotoxicity against melanoma and carcinoma cell lines; however, the active compounds responsible for this cytotoxicity have not been identified. A phytochemical investigation was conducted on <i>A. chinense</i> bulbs to identify novel anticancer seeds from natural products. Seven steroidal glycosides (<b>1–7</b>), comprising three spirostan-type (<b>1–3</b>) and four furostan-type (<b>4–7</b>), were obtained. This is the first report of the isolation of <b>6</b> and <b>7</b> from <i>A. chinese</i>. The cytotoxicity of <b>1–7</b> was evaluated in SBC-3 human small-cell lung cancer cells using the MTT assay. Compounds <b>2</b>, <b>3</b>, and <b>5</b> demonstrated moderate cytotoxicity against SBC-3 cells, with IC<sub>50</sub> values ranging from 15 to 42&#xa0;μM dose-dependently. Compound <b>3</b>, (25<i>R</i>)-3β-[(<i>O</i>-α-L-arabinopyranosyl-(1 → 6)-<i>O</i>-[β-D-xylopyranosyl-(1 → 4)]-β-D-glucopyranosyl)oxy]-5α-spirostan-6-one, which exhibited the most potent cytotoxicity among the isolated compounds, induced caspase-dependent apoptotic cell death via both mitochondrial dysfunction leading to reactive oxygen species (ROS) release and endoplasmic reticulum stress caused by ROS. Furthermore, since calreticulin exposure on the outer leaflet of the plasma membrane and extracellular ATP release were observed in SBC-3 cells treated with <b>3</b>, it may elicit immunogenic cell death in SBC-3 cells.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Apoptosis-inducing activity of a steroidal glycoside from Allium chinense G. Don. bulbs in human small-cell lung cancer cells via mitochondrial dysfunction and ER stress

  • 期刊论文

  • 发表日期：

    2025年7月12日

查看全文
  • Tamami Shimazaki,
  • Tomoki Iguchi,
  • Aki Kanda,
  • Ami Shirakawa,
  • Yoshihiro Mimaki

摘要

A saponin fraction from Allium chinense G. Don. bulbs has previously been reported to exhibit cytotoxicity against melanoma and carcinoma cell lines; however, the active compounds responsible for this cytotoxicity have not been identified. A phytochemical investigation was conducted on A. chinense bulbs to identify novel anticancer seeds from natural products. Seven steroidal glycosides (1–7), comprising three spirostan-type (1–3) and four furostan-type (4–7), were obtained. This is the first report of the isolation of 6 and 7 from A. chinese. The cytotoxicity of 1–7 was evaluated in SBC-3 human small-cell lung cancer cells using the MTT assay. Compounds 2, 3, and 5 demonstrated moderate cytotoxicity against SBC-3 cells, with IC50 values ranging from 15 to 42 μM dose-dependently. Compound 3, (25R)-3β-[(O-α-L-arabinopyranosyl-(1 → 6)-O-[β-D-xylopyranosyl-(1 → 4)]-β-D-glucopyranosyl)oxy]-5α-spirostan-6-one, which exhibited the most potent cytotoxicity among the isolated compounds, induced caspase-dependent apoptotic cell death via both mitochondrial dysfunction leading to reactive oxygen species (ROS) release and endoplasmic reticulum stress caused by ROS. Furthermore, since calreticulin exposure on the outer leaflet of the plasma membrane and extracellular ATP release were observed in SBC-3 cells treated with 3, it may elicit immunogenic cell death in SBC-3 cells.

Graphical abstract