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Purpose <p>This study investigates the ecological dynamics and functional responses of soil microbial communities under glyphosate stress. It compares two remediation strategies: biosolid amendments and bioaugmentation with plant growth-promoting bacteria (PGPB), namely <i>E. indicum</i> AS03, <i>R. rhodochrous</i> AS33, and <i>K. turfanensis</i> AS41, all isolated from bioaerosols. The main research questions are: (1) How do biosolids and PGPB bioaugmentation affect glyphosate degradation efficiency in soil? (2) How do these strategies influence microbial community, resilience under glyphosate exposure, and the ecological implications of these treatments for microbial biogeochemical cycles involved in glyphosate degradation?</p> Methods <p>A 60-day soil microcosm experiment was conducted using glyphosate-contaminated soils (40, 60, and 100&#xa0;mg kg<sup>-1</sup>), with biosolids (sterilized or non-sterilized) and bioaugmentation treatments. Community composition and functional responses were assessed via 16&#xa0;S rRNA sequencing, SPARCC network analysis, and quantification of genes related to carbon, nitrogen, and phosphorus cycling (e.g., <i>gltA</i>, <i>nxrA</i>, <i>phnI</i>, <i>phnJ</i>, <i>mpd1</i>, <i>mpd2</i>). Glyphosate and AMPA concentrations were monitored over time.</p> Results <p>Glyphosate significantly altered microbial community structure, favoring tolerant genera such as <i>Rhodococcus</i> and <i>Burkholderia</i>. Bioaugmentation was associated with increased microbial network connectivity and suggested a shift in metabolic potential, particularly in pathways related to carbon and nitrogen cycling. Sterilized biosolids, while promoting some degradation markers, significantly reduced microbial diversity (<i>P</i> &lt; 0.05) and functional redundancy.</p> Conclusions <p>Bioaugmentation supports microbial resilience and functional diversity under glyphosate stress. Conversely, sterilized biosolids, despite enhancing degradation indicators, may limit long-term microbial functionality. These results highlight the need to balance remediation effectiveness with microbial ecosystem stability in glyphosate-impacted soils. This study is among the first to evaluate airborne plant growth-promoting bacteria (PGPB) for glyphosate bioremediation, representing a novel and underexplored source of microbes adapted to harsh environmental conditions.</p>

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Comparative ecological change analysis in soil glyphosate degradation: biosolid amendment vs. bioaugmentation

  • 期刊论文

  • 发表日期：

    2026年1月22日

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  • Beatriz G. Guardado-Fierros,
  • Diego A. Tuesta-Popolizio,
  • Miguel A. Lorenzo-Santiago,
  • Thiago Gumiere,
  • Lydia Aid,
  • Jacobo Rodriguez-Campos,
  • Silvia Maribel Contreras-Ramos

摘要

Purpose

This study investigates the ecological dynamics and functional responses of soil microbial communities under glyphosate stress. It compares two remediation strategies: biosolid amendments and bioaugmentation with plant growth-promoting bacteria (PGPB), namely E. indicum AS03, R. rhodochrous AS33, and K. turfanensis AS41, all isolated from bioaerosols. The main research questions are: (1) How do biosolids and PGPB bioaugmentation affect glyphosate degradation efficiency in soil? (2) How do these strategies influence microbial community, resilience under glyphosate exposure, and the ecological implications of these treatments for microbial biogeochemical cycles involved in glyphosate degradation?

Methods

A 60-day soil microcosm experiment was conducted using glyphosate-contaminated soils (40, 60, and 100 mg kg-1), with biosolids (sterilized or non-sterilized) and bioaugmentation treatments. Community composition and functional responses were assessed via 16 S rRNA sequencing, SPARCC network analysis, and quantification of genes related to carbon, nitrogen, and phosphorus cycling (e.g., gltA, nxrA, phnI, phnJ, mpd1, mpd2). Glyphosate and AMPA concentrations were monitored over time.

Results

Glyphosate significantly altered microbial community structure, favoring tolerant genera such as Rhodococcus and Burkholderia. Bioaugmentation was associated with increased microbial network connectivity and suggested a shift in metabolic potential, particularly in pathways related to carbon and nitrogen cycling. Sterilized biosolids, while promoting some degradation markers, significantly reduced microbial diversity (P < 0.05) and functional redundancy.

Conclusions

Bioaugmentation supports microbial resilience and functional diversity under glyphosate stress. Conversely, sterilized biosolids, despite enhancing degradation indicators, may limit long-term microbial functionality. These results highlight the need to balance remediation effectiveness with microbial ecosystem stability in glyphosate-impacted soils. This study is among the first to evaluate airborne plant growth-promoting bacteria (PGPB) for glyphosate bioremediation, representing a novel and underexplored source of microbes adapted to harsh environmental conditions.