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Purpose <p>Doxycycline (DTX) and zinc (Zn) are widely used as feed additives to promote the growth of livestock and poultry, reduce morbidity, and lower mortality rates; however, their metabolic rates in animal bodies are low, and most of them are excreted with metabolic products. Currently, there is limited research on the effects of doxycycline and zinc on soil microbial diversity and the presence of resistance genes.</p> Materials and methods <p>The pot experiment was conducted combined with 16&#xa0;S sequencing analysis to elucidate the effects of composite pollution of DOX and Zn on thw growth of lettuce (two varieties, ‘Green Luosha’ and ‘Green Feicui’), soil enzymes, and soil microbial diversity.</p> Results and discussion <p>The edible (shoot) biomass of ‘Green Luosha’ lettuce and ‘Green Feicui’ lettuce decreased by 21.56%–54.63% and 5.46%–39.62% exposed to single and composite pollution of DOX and Zn, respectively. Among the two varieties, ‘Green Luosha’ lettuce was more sensitive to the stress response. Composite pollution of DOX and Zn significantly reduced the uptake of Zn by lettuce but promoted the uptake of DOX. Composite pollution of DOX and Zn increased soil microbial abundance and diversity, and the DOX content was extremely significantly negatively correlated with the relative abundance of the phylum Bacteroidota and the genus Bacillus in soil.</p> Conclusions <p>Both single and composite pollution of DOX and Zn significantly inhibited lettuce growth, and the inhibitory effect under composite pollution was stronger. Single or composite pollution of Zn and DOX reduced the activities of soil β-glucosidase and urease, and the inhibitory effect of composite pollution was greater than that of single pollution. The results of this study is of great value for the management and remediation of DOX and Zn pollution in soils.</p>

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Effects of doxycycline and zinc composite pollution on soil enzymes and microbial diversity

  • 期刊论文

  • 发表日期：

    2025年7月22日

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  • Jiangjie He,
  • Lanqin Wang,
  • Yurou Han,
  • Weihong Xu

摘要

Purpose

Doxycycline (DTX) and zinc (Zn) are widely used as feed additives to promote the growth of livestock and poultry, reduce morbidity, and lower mortality rates; however, their metabolic rates in animal bodies are low, and most of them are excreted with metabolic products. Currently, there is limited research on the effects of doxycycline and zinc on soil microbial diversity and the presence of resistance genes.

Materials and methods

The pot experiment was conducted combined with 16 S sequencing analysis to elucidate the effects of composite pollution of DOX and Zn on thw growth of lettuce (two varieties, ‘Green Luosha’ and ‘Green Feicui’), soil enzymes, and soil microbial diversity.

Results and discussion

The edible (shoot) biomass of ‘Green Luosha’ lettuce and ‘Green Feicui’ lettuce decreased by 21.56%–54.63% and 5.46%–39.62% exposed to single and composite pollution of DOX and Zn, respectively. Among the two varieties, ‘Green Luosha’ lettuce was more sensitive to the stress response. Composite pollution of DOX and Zn significantly reduced the uptake of Zn by lettuce but promoted the uptake of DOX. Composite pollution of DOX and Zn increased soil microbial abundance and diversity, and the DOX content was extremely significantly negatively correlated with the relative abundance of the phylum Bacteroidota and the genus Bacillus in soil.

Conclusions

Both single and composite pollution of DOX and Zn significantly inhibited lettuce growth, and the inhibitory effect under composite pollution was stronger. Single or composite pollution of Zn and DOX reduced the activities of soil β-glucosidase and urease, and the inhibitory effect of composite pollution was greater than that of single pollution. The results of this study is of great value for the management and remediation of DOX and Zn pollution in soils.