Scholarlink

<p>Originally standardized biodegradation tests, designed for small molecules, are often considered inadequate for evaluating polymers, as they do not account for the crucial fragmentation step catalyzed by exoenzymes, which is essential for initiating the microbial degradation of polymers. In this study, the presence of EEs in the environment and their role in the biodegradation of guar gum, a WSP, were evaluated. A metagenomic shotgun approach assessed the abundance of genes encoding <i>α-</i>galactosidase and <i>β</i>-mannosidase in the Waste Water Treatment Plant (WWTP) and soil. Secondly, we employed sonication to release EEs, measured their activity, and evaluated the impact of their release on guar degradation using biodegradation tests. These findings indicated that genes encoding <i>α-</i>galactosidase and <i>β</i>-mannosidase were more abundant in the WWTP than in soil. A sonication protocol with a power density of 1 W/mL and a frequency of 20&#xa0;kHz for 1&#xa0;min (WWTP) and 2&#xa0;min (soil) released EEs without significant cell lysis and significantly reduced the lag phase of guar-grafted acrylamide biodegradation from 8 to 5&#xa0;days and decreased variability in native guar biodegradation by 10%. Our findings highlight the potential of WWTP and soil microbial communities to degrade guar gum by screening EEs and propose a new method for preparing the inoculum for WSPs biodegradation tests.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Screening of exoenzymes for guar gum biodegradation in activated sludge and soil

  • 期刊论文

  • 发表日期：

    2026年1月5日

查看全文
  • Sepehr Aghajani,
  • Margaux Kerdraon,
  • James Wilson,
  • Sophie Galinat,
  • Corinne André-Miral,
  • Mickaël Cregut,
  • Sulivan Jouanneau,
  • Gérald Thouand

摘要

Originally standardized biodegradation tests, designed for small molecules, are often considered inadequate for evaluating polymers, as they do not account for the crucial fragmentation step catalyzed by exoenzymes, which is essential for initiating the microbial degradation of polymers. In this study, the presence of EEs in the environment and their role in the biodegradation of guar gum, a WSP, were evaluated. A metagenomic shotgun approach assessed the abundance of genes encoding α-galactosidase and β-mannosidase in the Waste Water Treatment Plant (WWTP) and soil. Secondly, we employed sonication to release EEs, measured their activity, and evaluated the impact of their release on guar degradation using biodegradation tests. These findings indicated that genes encoding α-galactosidase and β-mannosidase were more abundant in the WWTP than in soil. A sonication protocol with a power density of 1 W/mL and a frequency of 20 kHz for 1 min (WWTP) and 2 min (soil) released EEs without significant cell lysis and significantly reduced the lag phase of guar-grafted acrylamide biodegradation from 8 to 5 days and decreased variability in native guar biodegradation by 10%. Our findings highlight the potential of WWTP and soil microbial communities to degrade guar gum by screening EEs and propose a new method for preparing the inoculum for WSPs biodegradation tests.

Graphical Abstract