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<p>Scallops (<i>Mizuhopecten yessoensis</i>) accumulate abnormal levels of cadmium (Cd) in their bodies; however, the chemical form of Cd in vivo remains unclear. In this study, Cd concentrations in various scallop tissues were measured, revealing that Cd predominantly accumulated as a water-soluble component within the midgut gland. High-performance liquid chromatography combined with the post-column tetraphenylporphyrin tetrasulfonic acid (TPPS) experiment revealed that the molecular weight of the Cd-binding substances was approximately 44&#xa0;kDa. Moreover, Cd-immobilized affinity chromatography and CdS precipitation identified <i>M. yessoensis</i> meprin A subunit alpha-like (myMEP1A) as a Cd-binding protein. The myMEP1A gene was specifically expressed in the midgut gland. TPPS measurements indicated that myMEP1A exhibited strong Cd-binding activity. These results suggested that myMEP1A is&#xa0;a protein responsible for Cd accumulation in the midgut gland.</p>

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Exploring cadmium-binding proteins in Japanese scallops Mizuhopecten yessoensis: isolation and characterization

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Zehua Zheng,
  • Yuto Namikawa,
  • Yugo Kato,
  • Peng Lu,
  • Lumi Negishi,
  • Hitoshi Kurumizaka,
  • Koji Nagata,
  • Michio Suzuki

摘要

Scallops (Mizuhopecten yessoensis) accumulate abnormal levels of cadmium (Cd) in their bodies; however, the chemical form of Cd in vivo remains unclear. In this study, Cd concentrations in various scallop tissues were measured, revealing that Cd predominantly accumulated as a water-soluble component within the midgut gland. High-performance liquid chromatography combined with the post-column tetraphenylporphyrin tetrasulfonic acid (TPPS) experiment revealed that the molecular weight of the Cd-binding substances was approximately 44 kDa. Moreover, Cd-immobilized affinity chromatography and CdS precipitation identified M. yessoensis meprin A subunit alpha-like (myMEP1A) as a Cd-binding protein. The myMEP1A gene was specifically expressed in the midgut gland. TPPS measurements indicated that myMEP1A exhibited strong Cd-binding activity. These results suggested that myMEP1A is a protein responsible for Cd accumulation in the midgut gland.