Scholarlink

Background <p>Polyhydroxyalkanoates (PHA) have become biodegradable alternatives for replacing chemical polymers. However, high production costs remain the primary obstacle to the commercial application of this bioprocess, prompting the use of metabolic engineering approaches to optimize PHA quality and increase productivity.</p> Objectives <p>This study aimed to identify the endometabolome at different stages during a dynamic fermentation of <i>Burkholderia cepacia</i>using oleic acid as the carbon source.</p> Methodology <p>Untargeted metabolomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) techniques. Univariate (UVA) and multivariate (MVA) statistical analyses were performed to determine significant differences between metabolomic profiles.</p> Results <p>A total of 24 significant metabolites were identified through GC-MS analysis and 223 through LC-MS, highlighting organic compounds and lipids associated with pathways such as ß-oxidation, the tricarboxylic acid cycle, and the pentose phosphate pathway. Acetyl-CoA emerged as a critical common intermediate in both central biosynthetic and PHA-producing pathways.</p> Conclusion <p>Acetyl-CoA plays a fundamental role in the complex regulatory system activated by nutrient fluctuations in the culture medium, suggesting that the microorganism undergoes metabolic reorganization to optimize carbon source utilization for cellular maintenance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metabolomic characterization and temporal dynamics of Burkholderia cepacia for polyhydroxyalkanoates production from oleic acid

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月16日

查看全文
  • Alfonso E. Alarcón,
  • Nubia C. Moreno,
  • Daniel Pardo-Rodriguez,
  • Mónica P. Cala,
  • Carlos A. M. Riascos

摘要

Background

Polyhydroxyalkanoates (PHA) have become biodegradable alternatives for replacing chemical polymers. However, high production costs remain the primary obstacle to the commercial application of this bioprocess, prompting the use of metabolic engineering approaches to optimize PHA quality and increase productivity.

Objectives

This study aimed to identify the endometabolome at different stages during a dynamic fermentation of Burkholderia cepaciausing oleic acid as the carbon source.

Methodology

Untargeted metabolomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) techniques. Univariate (UVA) and multivariate (MVA) statistical analyses were performed to determine significant differences between metabolomic profiles.

Results

A total of 24 significant metabolites were identified through GC-MS analysis and 223 through LC-MS, highlighting organic compounds and lipids associated with pathways such as ß-oxidation, the tricarboxylic acid cycle, and the pentose phosphate pathway. Acetyl-CoA emerged as a critical common intermediate in both central biosynthetic and PHA-producing pathways.

Conclusion

Acetyl-CoA plays a fundamental role in the complex regulatory system activated by nutrient fluctuations in the culture medium, suggesting that the microorganism undergoes metabolic reorganization to optimize carbon source utilization for cellular maintenance.