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Introduction <p>LC-MS system suitability test (SST) is crucial for reliable data acquisition especially in untargeted metabolomics.</p> Objectives <p>Identification of best reference materials (RMs) to improve best quality assurance (QA) and quality control (QC) practices.</p> Methods <p>Investigations were performed using a C<sub>18</sub> reversed-phase (RP) column LC-MS approach.</p> Results <p>Targeted cyanotoxin analysis revealed a performance loss of the used C<sub>18</sub> RP column although the SST confirmed a fit for purpose instrument which prompted to test several additional RMs.</p> Conclusion <p>QA procedures for LC-MS can be improved by incorporating polar microcystins or arginine methyl ester as RMs for SST.</p>

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Polar microcystins or arginine methyl ester can serve as sensitive reference materials for system suitability tests in untargeted metabolomics using reversed-phase LC-MS: A case study

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  • 发表日期：

    2026年3月7日

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  • Tommy Melzer,
  • Georg Pohnert,
  • Nico Ueberschaar

摘要

Introduction

LC-MS system suitability test (SST) is crucial for reliable data acquisition especially in untargeted metabolomics.

Objectives

Identification of best reference materials (RMs) to improve best quality assurance (QA) and quality control (QC) practices.

Methods

Investigations were performed using a C18 reversed-phase (RP) column LC-MS approach.

Results

Targeted cyanotoxin analysis revealed a performance loss of the used C18 RP column although the SST confirmed a fit for purpose instrument which prompted to test several additional RMs.

Conclusion

QA procedures for LC-MS can be improved by incorporating polar microcystins or arginine methyl ester as RMs for SST.