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Introduction <p>Altered metabolic pathways are critical in the progression of traumatic brain injury (TBI). Identifying differentially abundant metabolites (DAMs) from specific cell types can offer valuable diagnostic and prognostic insights.</p> Objective <p>This study aimed to characterize the metabolomic profile of injured human brain microvascular endothelial cells (hBMEC) at 2-, 12-, 24-, and 48&#xa0;h post-injury.</p> Methods <p>Using an in vitro TBI model, we analyzed metabolites in cell culture media through a combination of direct injection mass spectrometry and a custom reverse-phase LC–MS/MS assay. We evaluated 644 metabolites at each time point.</p> Results <p>Phosphatidylcholines were significantly upregulated across all time intervals. At 2- and 12&#xa0;h post-injury, the most significantly upregulated metabolites included sphingomyelin (OH) C22:1, ethylmalonic acid, and methylhistidine, while guanosine and the combination of butyric acid + isobutyric acid were the most downregulated. At 24 and 48&#xa0;h, deoxyadenosine and inosine, respectively, emerged as the most upregulated metabolites, with butyric acid + isobutyric acid and quinoline-4-carboxylic acid showing the greatest downregulation.</p> Conclusion <p>Metabolomic profiling identified various DAMs after traumatic injury that are linked to human endothelial dysfunction. Future experiments should expand the number of metabolites measured to determine the underlying signaling pathways.</p>

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Metabolomic alterations in human brain microvascular endothelial cells induced by traumatic injury

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月27日

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  • Enis Cela,
  • David Tweddell,
  • Eric K. Patterson,
  • Mark Daley,
  • Gediminas Cepinskas,
  • Douglas D. Fraser

摘要

Introduction

Altered metabolic pathways are critical in the progression of traumatic brain injury (TBI). Identifying differentially abundant metabolites (DAMs) from specific cell types can offer valuable diagnostic and prognostic insights.

Objective

This study aimed to characterize the metabolomic profile of injured human brain microvascular endothelial cells (hBMEC) at 2-, 12-, 24-, and 48 h post-injury.

Methods

Using an in vitro TBI model, we analyzed metabolites in cell culture media through a combination of direct injection mass spectrometry and a custom reverse-phase LC–MS/MS assay. We evaluated 644 metabolites at each time point.

Results

Phosphatidylcholines were significantly upregulated across all time intervals. At 2- and 12 h post-injury, the most significantly upregulated metabolites included sphingomyelin (OH) C22:1, ethylmalonic acid, and methylhistidine, while guanosine and the combination of butyric acid + isobutyric acid were the most downregulated. At 24 and 48 h, deoxyadenosine and inosine, respectively, emerged as the most upregulated metabolites, with butyric acid + isobutyric acid and quinoline-4-carboxylic acid showing the greatest downregulation.

Conclusion

Metabolomic profiling identified various DAMs after traumatic injury that are linked to human endothelial dysfunction. Future experiments should expand the number of metabolites measured to determine the underlying signaling pathways.