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Introduction <p>Faecal amino acids are promising non-invasive diagnostic biomarkers, but stability remains unclear.</p> Objectives <p>This study examined whether storage conditions, sampling site within the sample, freeze-thaw cycles, and the OMNImet<sup>®</sup>·GUT device affect faecal amino acid concentrations.</p> Methods <p>Faecal samples from three donors underwent various pre-analytical conditions. Amino acids were analysed through targeted liquid chromatography-tandem mass spectrometry.</p> Results <p>Most amino acids remained stable across sampling sites and freeze-thaw cycles. Storage at -20&#xa0;°C preserved integrity, whereas 4&#xa0;°C and 20&#xa0;°C storage led to variations. The OMNImet<sup>®</sup>·GUT device stabilised some amino acids but showed inconsistencies.</p> Conclusion <p>Pre-analytical conditions influence faecal amino acid concentrations. Standardisation is essential for biomarker reliability.</p>

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Faecal amino acids stability: investigating optimal sampling conditions for analysis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月14日

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  • Roza C. M. Opperman,
  • Eva Vermeer,
  • Sofie Bosch,
  • Tim G. J. de Meij,
  • Nanne K. H. de Boer,
  • Eduard A. Struys

摘要

Introduction

Faecal amino acids are promising non-invasive diagnostic biomarkers, but stability remains unclear.

Objectives

This study examined whether storage conditions, sampling site within the sample, freeze-thaw cycles, and the OMNImet®·GUT device affect faecal amino acid concentrations.

Methods

Faecal samples from three donors underwent various pre-analytical conditions. Amino acids were analysed through targeted liquid chromatography-tandem mass spectrometry.

Results

Most amino acids remained stable across sampling sites and freeze-thaw cycles. Storage at -20 °C preserved integrity, whereas 4 °C and 20 °C storage led to variations. The OMNImet®·GUT device stabilised some amino acids but showed inconsistencies.

Conclusion

Pre-analytical conditions influence faecal amino acid concentrations. Standardisation is essential for biomarker reliability.