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Introduction <p>The <i>ANGUSTIFOLIA3</i> (<i>AN3</i>) gene encodes a transcriptional co-activator for cell proliferation in <i>Arabidopsis thaliana</i> leaves. We previously showed that <i>Physcomitrium patens AN3</i> orthologs promote gametophore shoot formation through arginine metabolism.</p> Objectives <p>We analyzed the role of <i>AN3</i> in <i>Arabidopsis thaliana</i> to understand how seedling growth is regulated by metabolic and physiological modulations.</p> Methods <p>We first explored amino acids that affect the seedling growth of <i>an3</i> mutants. Transcriptome and metabolome analyses were conducted to elucidate the metabolic and physiological roles of <i>AN3</i> during seedling growth. Lastly, we examined the distribution of reactive oxygen species to corroborate our omics-based findings.</p> Results <p>Our results indicated that <i>an3</i> mutants were unable to establish seedlings when grown with leucine, but not arginine. Multi-omics analyses suggested that <i>an3</i> mutants exhibit a hypoxia-like response. Abnormal oxidative status was confirmed by detecting an altered distribution of reactive oxygen species in the roots of <i>an3</i> mutants.</p> Conclusion <p><i>AN3</i> helps maintain the leucine metabolism and oxidative balance during seedling growth in <i>Arabidopsis thaliana</i>. Future research is necessary to explore the interaction between these processes.</p>

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Loss-of-functional mutation in ANGUSTIFOLIA3 causes leucine hypersensitivity and hypoxia response during Arabidopsis thaliana seedling growth

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月31日

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  • Kensuke Kawade,
  • Mamoru Nozaki,
  • Gorou Horiguchi,
  • Tomoko Mori,
  • Katsushi Yamaguchi,
  • Mami Okamoto,
  • Hiromitsu Tabeta,
  • Shuji Shigenobu,
  • Masami Yokota Hirai,
  • Hirokazu Tsukaya

摘要

Introduction

The ANGUSTIFOLIA3 (AN3) gene encodes a transcriptional co-activator for cell proliferation in Arabidopsis thaliana leaves. We previously showed that Physcomitrium patens AN3 orthologs promote gametophore shoot formation through arginine metabolism.

Objectives

We analyzed the role of AN3 in Arabidopsis thaliana to understand how seedling growth is regulated by metabolic and physiological modulations.

Methods

We first explored amino acids that affect the seedling growth of an3 mutants. Transcriptome and metabolome analyses were conducted to elucidate the metabolic and physiological roles of AN3 during seedling growth. Lastly, we examined the distribution of reactive oxygen species to corroborate our omics-based findings.

Results

Our results indicated that an3 mutants were unable to establish seedlings when grown with leucine, but not arginine. Multi-omics analyses suggested that an3 mutants exhibit a hypoxia-like response. Abnormal oxidative status was confirmed by detecting an altered distribution of reactive oxygen species in the roots of an3 mutants.

Conclusion

AN3 helps maintain the leucine metabolism and oxidative balance during seedling growth in Arabidopsis thaliana. Future research is necessary to explore the interaction between these processes.