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<p>The human P2X7 purinergic receptor is an ATP-gated ion channel expressed across a wide range of cell types, including immune, epithelial, glial, and various cancer cells. Despite its broad expression and diverse functions, little is known about the transcriptional regulation of the <i>P2RX7</i> gene. In this study, the transcription initiation site of the <i>P2RX7</i> gene was mapped 149/148 nucleotides upstream of the ATG start codon by using a sequencing-based primer extension method. To characterise the promoter region of the <i>P2RX7</i> gene, eight DNA fragments were cloned into a dual-colour luciferase reporter vector system. The promoter activity of each fragment was assessed in transfected HEK-293 and COS-7 cells. Predicted promoter binding sequences were identified, and fluorescence-based super-electrophoretic mobility shift assay confirmed that the transcription factors YY1 and C/EBPβ directly bind to the <i>P2RX7</i> promoter region.</p>

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Identification of the promoter region of the human P2RX7 gene

  • 期刊论文

  • 发表日期：

    2026年5月9日

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  • Luyao Liu,
  • Yoshiteru Kagawa,
  • Colin L. Masters,
  • Stanley S. Stylli,
  • Theo Mantamadiotis,
  • Ben J. Gu

摘要

The human P2X7 purinergic receptor is an ATP-gated ion channel expressed across a wide range of cell types, including immune, epithelial, glial, and various cancer cells. Despite its broad expression and diverse functions, little is known about the transcriptional regulation of the P2RX7 gene. In this study, the transcription initiation site of the P2RX7 gene was mapped 149/148 nucleotides upstream of the ATG start codon by using a sequencing-based primer extension method. To characterise the promoter region of the P2RX7 gene, eight DNA fragments were cloned into a dual-colour luciferase reporter vector system. The promoter activity of each fragment was assessed in transfected HEK-293 and COS-7 cells. Predicted promoter binding sequences were identified, and fluorescence-based super-electrophoretic mobility shift assay confirmed that the transcription factors YY1 and C/EBPβ directly bind to the P2RX7 promoter region.