Scholarlink

<p>Soil salinity severely impairs crop growth and yield by inducing ion toxicity, osmotic stress, and disrupting plant nutrient uptake. This pervasive stressor poses a major threat to global cereal production and food security, especially the wheat crop. Plant growth-promoting rhizobacteria (PGPR) capable of producing 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) and exopolysaccharides (EPS) offer a promising biological strategy to enhance plant tolerance under saline conditions. This study evaluated the combined application of halotolerant ACCD- and EPS-producing bacterial strains to mitigate salt stress (150 mM NaCl) in wheat. Eight strains were selected based on functional traits: ACCD producers (sz18 <i>Brevibacterium frigoritolerans</i>, sz30 <i>Bacillus spizizienii</i>, sz35 <i>Pseudomonas glycinis</i>, sz80 <i>Pseudomonas grimontii</i>) and EPS producers (EP5 <i>Bacillus tequilensis</i>, EP8 <i>Bacillus spizizienii</i>, EP29 <i>Pseudomonas koreensis</i>, EP35 <i>Bacillus subtilis</i>). All strains successfully colonized the wheat rhizosphere. Co-inoculation of ACCD and EPS-producing bacteria significantly enhanced seedling performance under salinity, increasing fresh biomass and root and shoot length by approximately 30% compared with uninoculated controls. Gene expression analysis further demonstrated that inoculated plants exhibited substantial upregulation of key salt-responsive genes: <i>rbcS</i> (~ 3-fold), <i>rbcL</i> (~ 6-fold), <i>cAPX</i> (~ 6-fold), and <i>DREB2</i> (~ 2-fold). These transcriptional changes indicate improved photosynthetic capacity, antioxidant defense, and stress-responsive regulation in treated plants. Overall, the findings highlight the synergistic potential of ACCD- and EPS-producing PGPR in alleviating salinity stress in wheat. If validated under field conditions, this microbial consortium could serve as an effective, sustainable approach to improve wheat resilience and productivity in salt-affected soils.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Plant growth-promoting rhizobacteria (PGPR) producing ACC deaminase and exopolysaccharides enhance salt tolerance in wheat

  • 期刊论文

  • 发表日期：

    2026年7月27日

查看全文
  • Mahnoor Hayat,
  • Rashida Parveen,
  • Muhammad Shoib Nawaz,
  • Muhammad Ahmad Rao,
  • Ahmed Waqar,
  • Rubab Zahra. Naqvi,
  • Munir Ahmad Anwar,
  • Zahir Ahmad Zahir,
  • Muhammad Qasim,
  • M Ahmad Dogar,
  • Tadeo Sáez Sandino,
  • Brajesh K. Singh,
  • Asma Imran

摘要

Soil salinity severely impairs crop growth and yield by inducing ion toxicity, osmotic stress, and disrupting plant nutrient uptake. This pervasive stressor poses a major threat to global cereal production and food security, especially the wheat crop. Plant growth-promoting rhizobacteria (PGPR) capable of producing 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) and exopolysaccharides (EPS) offer a promising biological strategy to enhance plant tolerance under saline conditions. This study evaluated the combined application of halotolerant ACCD- and EPS-producing bacterial strains to mitigate salt stress (150 mM NaCl) in wheat. Eight strains were selected based on functional traits: ACCD producers (sz18 Brevibacterium frigoritolerans, sz30 Bacillus spizizienii, sz35 Pseudomonas glycinis, sz80 Pseudomonas grimontii) and EPS producers (EP5 Bacillus tequilensis, EP8 Bacillus spizizienii, EP29 Pseudomonas koreensis, EP35 Bacillus subtilis). All strains successfully colonized the wheat rhizosphere. Co-inoculation of ACCD and EPS-producing bacteria significantly enhanced seedling performance under salinity, increasing fresh biomass and root and shoot length by approximately 30% compared with uninoculated controls. Gene expression analysis further demonstrated that inoculated plants exhibited substantial upregulation of key salt-responsive genes: rbcS (~ 3-fold), rbcL (~ 6-fold), cAPX (~ 6-fold), and DREB2 (~ 2-fold). These transcriptional changes indicate improved photosynthetic capacity, antioxidant defense, and stress-responsive regulation in treated plants. Overall, the findings highlight the synergistic potential of ACCD- and EPS-producing PGPR in alleviating salinity stress in wheat. If validated under field conditions, this microbial consortium could serve as an effective, sustainable approach to improve wheat resilience and productivity in salt-affected soils.