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<p>Sulfides accumulation during anaerobic digestion negatively affects biogas quality, process stability, and infrastructure integrity, making efficient desulfurization strategies essential. This study investigated sulfur-oxidizing bacterial communities associated with anaerobic digesters and a Thiopaq® bioreactor at the Marrakech wastewater treatment plant. High-throughput 16S rRNA gene sequencing revealed a marked ecological contrast between the two systems. While the Thiopaq® bioreactor was largely dominated by a single <i>Thioalkalibacteraceae</i> lineage, anaerobic sludge environments harbored a phylogenetically diverse assemblage of sulfur-oxidizing bacteria spanning multiple proteobacterial families, including <i>Thiobacillaceae</i>, <i>Rhodobacteraceae</i>, <i>Hyphomicrobiaceae</i>, <i>Paracoccaceae</i>, <i>Comamonadaceae</i>, <i>Rhodocyclaceae</i>, <i>Zoogloeaceae</i>, <i>Azonexaceae</i>, and <i>Burkholderiaceae</i>. Phylogenetic reconstruction and culture-based isolation showed only partial overlap between sequencing-derived operational taxonomic units and cultivated strains, highlighting the complementarity of molecular and physiological approaches. Functional assays demonstrated that several indigenous isolates were capable of oxidizing sulfide produced by sulfate-reducing bacteria under both microaerophilic and nitrate-reducing conditions. Sulfide concentrations decreased from 14.87&#xa0;mM to 0.03&#xa0;mM, corresponding to a removal efficiency of 99.8%, under denitrifying conditions and accompanied by elemental sulfur formation. These findings indicate that locally adapted sulfur-oxidizing bacteria constitute a functional reservoir that can be exploited for improved sulfide management. The results support the development of site-specific strategies, including controlled microaeration within anaerobic digesters and the use of indigenous inocula for biological desulfurization, as alternatives to exclusive reliance on commercial strains.</p>

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Diversity and functional potential of indigenous sulfur-oxidizing bacteria in anaerobic digesters and a Thiopaq bioreactor

  • 期刊论文

  • 发表日期：

    2026年7月6日

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  • Abdelaziz El Houari,
  • Magali Ranchou-Peyruse,
  • Anthony Ranchou-Peyruse,
  • Rhizlane Bennisse,
  • Radia Bouterfas,
  • Abdel-Illah Qatibi,
  • Rémy Guyoneaud

摘要

Sulfides accumulation during anaerobic digestion negatively affects biogas quality, process stability, and infrastructure integrity, making efficient desulfurization strategies essential. This study investigated sulfur-oxidizing bacterial communities associated with anaerobic digesters and a Thiopaq® bioreactor at the Marrakech wastewater treatment plant. High-throughput 16S rRNA gene sequencing revealed a marked ecological contrast between the two systems. While the Thiopaq® bioreactor was largely dominated by a single Thioalkalibacteraceae lineage, anaerobic sludge environments harbored a phylogenetically diverse assemblage of sulfur-oxidizing bacteria spanning multiple proteobacterial families, including Thiobacillaceae, Rhodobacteraceae, Hyphomicrobiaceae, Paracoccaceae, Comamonadaceae, Rhodocyclaceae, Zoogloeaceae, Azonexaceae, and Burkholderiaceae. Phylogenetic reconstruction and culture-based isolation showed only partial overlap between sequencing-derived operational taxonomic units and cultivated strains, highlighting the complementarity of molecular and physiological approaches. Functional assays demonstrated that several indigenous isolates were capable of oxidizing sulfide produced by sulfate-reducing bacteria under both microaerophilic and nitrate-reducing conditions. Sulfide concentrations decreased from 14.87 mM to 0.03 mM, corresponding to a removal efficiency of 99.8%, under denitrifying conditions and accompanied by elemental sulfur formation. These findings indicate that locally adapted sulfur-oxidizing bacteria constitute a functional reservoir that can be exploited for improved sulfide management. The results support the development of site-specific strategies, including controlled microaeration within anaerobic digesters and the use of indigenous inocula for biological desulfurization, as alternatives to exclusive reliance on commercial strains.