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<p>The aim of the present study was to improve survival rate of the probiotic strain <i>Lactiplantibacillus plantarum</i> BF_15 during industrial production and storage by supplementing the culture medium with glutathione (GSH). The addition of 3 mM GSH significantly improved the survival rate after freeze-drying (94.45 ± 1.10%), storage at room temperature (74.81 ± 0.19% at 25 ℃ for 8 weeks), and simulated digestion under gastrointestinal conditions (46.03 ± 5.73%) (<i>P</i> &lt; 0.05). GSH supplementation led to an increase in the relative proportion of unsaturated fatty acids in the cell membrane and superoxide dismutase (SOD) activity, while reducing the levels of intracellular reactive oxygen species (ROS). Moreover, GSH supplementation led to an upregulation of four genes related to redox homeostasis and DNA repair (i.e., <i>gshR</i>, <i>LTG66_RS00925</i>, <i>recA</i>, and <i>LTG66_RS14465</i>). These effects established a synergistic GSH-dependent defense network centered on antioxidant metabolism (SOD-GPx-GR system) and DNA protection (RecA-Dps system). Taken together, this study proposes a model for explaining the coordinated mechanisms by which glutathione mediates redox homeostasis and DNA repair in a probiotic strain, providing a sustainable, scalable, and low-carbon preservation strategy suited for industrial application.</p>

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Synergistic enhancement of industrial adaptability in Lactiplantibacillus plantarum BF_15 through glutathione-mediated redox and DNA repair pathways

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • Yalin Liu,
  • Na Zhang,
  • Aixin Guo,
  • Hongjie Liu,
  • Dongyao Li,
  • Chen Li,
  • Hongyan Kang,
  • Hongtao Tian

摘要

The aim of the present study was to improve survival rate of the probiotic strain Lactiplantibacillus plantarum BF_15 during industrial production and storage by supplementing the culture medium with glutathione (GSH). The addition of 3 mM GSH significantly improved the survival rate after freeze-drying (94.45 ± 1.10%), storage at room temperature (74.81 ± 0.19% at 25 ℃ for 8 weeks), and simulated digestion under gastrointestinal conditions (46.03 ± 5.73%) (P < 0.05). GSH supplementation led to an increase in the relative proportion of unsaturated fatty acids in the cell membrane and superoxide dismutase (SOD) activity, while reducing the levels of intracellular reactive oxygen species (ROS). Moreover, GSH supplementation led to an upregulation of four genes related to redox homeostasis and DNA repair (i.e., gshR, LTG66_RS00925, recA, and LTG66_RS14465). These effects established a synergistic GSH-dependent defense network centered on antioxidant metabolism (SOD-GPx-GR system) and DNA protection (RecA-Dps system). Taken together, this study proposes a model for explaining the coordinated mechanisms by which glutathione mediates redox homeostasis and DNA repair in a probiotic strain, providing a sustainable, scalable, and low-carbon preservation strategy suited for industrial application.