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<p>Sunflower stalk biomass (SSB), a xylan rich agro-residue may serve as an efficient substrate for prebiotics-xylooligosaccharides (XOS) production, offering significant application potential for food/pharmaceutical industries. Current study optimized a chemo-enzymatic bioprocess for XOS production from SSB using Design of Experiments, and the XOS were characterized for various biofunctional attributes. Optimization of delignification (1:1 acetic acid 8.74&#xa0;M : hydrogen peroxide 21.6&#xa0;M, at 2.07% w/v biomass loading, 44.16&#xa0;°C, 3.98&#xa0;h), followed by xylan extraction with NaOH (10.18% biomass loading, 59&#xa0;°C, and 6.50% NaOH), enhanced the xylan yield (2.44-fold). Purity and thermal stability of xylan were confirmed by FTIR and TGA, respectively. Xylan was hydrolyzed (2%, w/v xylan, 100 IU endoxylanase, 24&#xa0;h) using an <i>in-house</i> β-xylosidase-free endoxylanase from <i>Aspergillus flavus</i> MG-7 to produce XOS. A high XOS yield (91.37%, w/w) with varying degree of polymerization i.e. xylotetrose (66.53%, w/w), xylotriose (12.96%, w/w), and xylobiose (11.87%, w/w) was obtained. The XOS exhibited strong growth-promoting activity for the probiotic <i>Lactobacillus plantarum</i> M-13, LPM-13 (18.76 log CFU/ml), a positive prebiotic activity score (1.57 relative to <i>E. coli</i>), and significant antioxidant activity (DPPH free radical scavenging activity 88.47% at 5.0&#xa0;mg/ml). A varying antibacterial activity of cultural filtrate of LPM-13-grown modified MRS (glucose replaced with XOS, 1% w/v) was observed against <i>E. coli</i> 73.62%, <i>Klebsiella</i> sp. 68.24%, <i>P. aeruginosa</i> 64.29%, <i>E. faecalis</i> 57.42%, <i>B. cereus</i> 53.21%, <i>M. luteus</i> 51.73%, <i>S. pneumoniae</i> 47.53%, and <i>P. alcaligenes</i> 34.27%. Results suggest that XOS from SSB may have promising applications in the food, pharmaceutical, and nutraceutical industries.</p> Graphical Abstract <p></p>

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Integrated chemo-enzymatic strategy for production of functional xylooligosaccharides from sunflower stalk biomass

  • 期刊论文

  • 发表日期：

    2026年4月18日

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  • Mahak Gupta,
  • Ridhika Bangotra,
  • Surbhi Sharma,
  • Bisma Habib,
  • Arpana Thakur,
  • Priya Singh,
  • Muskaan Chib,
  • Parushi Nargotra,
  • Vishal Sharma,
  • Nisha Kapoor,
  • Ritu Mahajan,
  • Sumit G. Gandhi,
  • Bijender Kumar Bajaj

摘要

Sunflower stalk biomass (SSB), a xylan rich agro-residue may serve as an efficient substrate for prebiotics-xylooligosaccharides (XOS) production, offering significant application potential for food/pharmaceutical industries. Current study optimized a chemo-enzymatic bioprocess for XOS production from SSB using Design of Experiments, and the XOS were characterized for various biofunctional attributes. Optimization of delignification (1:1 acetic acid 8.74 M : hydrogen peroxide 21.6 M, at 2.07% w/v biomass loading, 44.16 °C, 3.98 h), followed by xylan extraction with NaOH (10.18% biomass loading, 59 °C, and 6.50% NaOH), enhanced the xylan yield (2.44-fold). Purity and thermal stability of xylan were confirmed by FTIR and TGA, respectively. Xylan was hydrolyzed (2%, w/v xylan, 100 IU endoxylanase, 24 h) using an in-house β-xylosidase-free endoxylanase from Aspergillus flavus MG-7 to produce XOS. A high XOS yield (91.37%, w/w) with varying degree of polymerization i.e. xylotetrose (66.53%, w/w), xylotriose (12.96%, w/w), and xylobiose (11.87%, w/w) was obtained. The XOS exhibited strong growth-promoting activity for the probiotic Lactobacillus plantarum M-13, LPM-13 (18.76 log CFU/ml), a positive prebiotic activity score (1.57 relative to E. coli), and significant antioxidant activity (DPPH free radical scavenging activity 88.47% at 5.0 mg/ml). A varying antibacterial activity of cultural filtrate of LPM-13-grown modified MRS (glucose replaced with XOS, 1% w/v) was observed against E. coli 73.62%, Klebsiella sp. 68.24%, P. aeruginosa 64.29%, E. faecalis 57.42%, B. cereus 53.21%, M. luteus 51.73%, S. pneumoniae 47.53%, and P. alcaligenes 34.27%. Results suggest that XOS from SSB may have promising applications in the food, pharmaceutical, and nutraceutical industries.

Graphical Abstract