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<p>Combinatorial engineering strategies were performed to improve cellulase production efficiencies of <i>Aspergillus niger</i>. Single functional cellulases in <i>Aspergillus niger</i> were knocked out with the exogenous multifunctional cellulases. Non-cellulase enzymes of phytase, β-mannanase and pectinase were deleted out from <i>Aspergillus niger.</i> The exogenous protein disulfide isomerase was expressed in <i>Aspergillus niger</i>. The cellulase activities from the reconstructed <i>Aspergillus niger</i> increased from 0.62 U/mL to 7.96 U/mL. Glucose released from corn stover increased from 12.5&#xa0;g/L to 27.8&#xa0;g/L. Deleting non-cellulase enzymes, expressing the multifunctional cellulase and exogenous protein disulfide isomerase could enhance cellulase production efficiencies of <i>Aspergillus niger</i>.</p>

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Combinatorial engineering strategies to reconstruct Aspergillus niger for improving its cellulase production efficiency

  • 期刊论文

  • 发表日期：

    2025年12月24日

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  • Dongsheng Xue,
  • Jueyan Chen,
  • Dejun Duan,
  • Zhiyong Chen,
  • Xihuo You

摘要

Combinatorial engineering strategies were performed to improve cellulase production efficiencies of Aspergillus niger. Single functional cellulases in Aspergillus niger were knocked out with the exogenous multifunctional cellulases. Non-cellulase enzymes of phytase, β-mannanase and pectinase were deleted out from Aspergillus niger. The exogenous protein disulfide isomerase was expressed in Aspergillus niger. The cellulase activities from the reconstructed Aspergillus niger increased from 0.62 U/mL to 7.96 U/mL. Glucose released from corn stover increased from 12.5 g/L to 27.8 g/L. Deleting non-cellulase enzymes, expressing the multifunctional cellulase and exogenous protein disulfide isomerase could enhance cellulase production efficiencies of Aspergillus niger.