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<p><i>Dunaliella parva</i> is halophilic green alga. <i>Dunaliella parva</i> may produce a large amount of starch, which has great practical value for bioethanol production. Glucose may be catalyzed by rate-limiting enzyme ADP-glucose pyrophosphorylase to produce starch. Our previous study cloned <i>DpAGPs</i> gene encoding ADP-glucose pyrophosphorylase (AGPase) small subunit from <i>Dunaliella parva</i> (<i>D. parva</i>). However, the function of <i>DpAGPs</i> remains unclear at present. To assess the influences of <i>DpAGPs</i> on metabolism of <i>D. parva</i>, this paper constructed recombinant plasmid pBI221-UbiΩ-CAT-AGPs, and transformed recombinant plasmid and empty plasmid pBI221-GFP-UbiΩ-CAT to <i>D. parva</i>. In transgenic <i>D. parva</i> overexpressing <i>DpAGPs</i> (sample D.parva T), both protein, carbohydrate and starch contents, AGPase activity and <i>DpAGPs</i> mRNA level increased compared with control sample D.parva C overexpressing the empty plasmid. Protein content of D.parva T (88.38&#xa0;mg/g dry weight) increased by 11.18&#xa0;mg/g DW compared with D.parva C (77.20&#xa0;mg/g dry weight) at 15 d. In particular, compared with sample D.parva C, starch contents sharply increased by 34.83&#xa0;mg/g dry weight (DW) and 53.51&#xa0;mg/g DW, and carbohydrate contents sharply increased by 143.24&#xa0;mg/g DW and 143.66&#xa0;mg/g DW at 15 d/18 d in <i>DpAGPs</i> transgenic D.parva T. However, pigments (chlorophyll a, chlorophyll b and carotenoid) and oil contents decreased in D.parva T. The absolute oil contents of D.parva T (23.97% and 21.79%) decreased by 25.40% and 27.68% compared with D.parva C (49.37% and 49.47%) at 15 d and 18 d, respectively. This paper observed the role of <i>DpAGPs</i> in <i>D. parva</i>, providing a reference for improving microalgal starch content and enhancing the practical value of <i>D. parva</i> for bioethanol production. The engineered <i>D. parva</i> with higher starch and carbohydrate contents had a great potential for bioethanol production.</p>

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DpAGPs overexpression enhanced application value of Dunaliella Parva for bioethanol production

  • 期刊论文

  • 发表日期：

    2026年1月6日

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  • Limei Huang,
  • Jin Zhang,
  • Yuwen Guo,
  • Shiyu Liang,
  • Changhua Shang

摘要

Dunaliella parva is halophilic green alga. Dunaliella parva may produce a large amount of starch, which has great practical value for bioethanol production. Glucose may be catalyzed by rate-limiting enzyme ADP-glucose pyrophosphorylase to produce starch. Our previous study cloned DpAGPs gene encoding ADP-glucose pyrophosphorylase (AGPase) small subunit from Dunaliella parva (D. parva). However, the function of DpAGPs remains unclear at present. To assess the influences of DpAGPs on metabolism of D. parva, this paper constructed recombinant plasmid pBI221-UbiΩ-CAT-AGPs, and transformed recombinant plasmid and empty plasmid pBI221-GFP-UbiΩ-CAT to D. parva. In transgenic D. parva overexpressing DpAGPs (sample D.parva T), both protein, carbohydrate and starch contents, AGPase activity and DpAGPs mRNA level increased compared with control sample D.parva C overexpressing the empty plasmid. Protein content of D.parva T (88.38 mg/g dry weight) increased by 11.18 mg/g DW compared with D.parva C (77.20 mg/g dry weight) at 15 d. In particular, compared with sample D.parva C, starch contents sharply increased by 34.83 mg/g dry weight (DW) and 53.51 mg/g DW, and carbohydrate contents sharply increased by 143.24 mg/g DW and 143.66 mg/g DW at 15 d/18 d in DpAGPs transgenic D.parva T. However, pigments (chlorophyll a, chlorophyll b and carotenoid) and oil contents decreased in D.parva T. The absolute oil contents of D.parva T (23.97% and 21.79%) decreased by 25.40% and 27.68% compared with D.parva C (49.37% and 49.47%) at 15 d and 18 d, respectively. This paper observed the role of DpAGPs in D. parva, providing a reference for improving microalgal starch content and enhancing the practical value of D. parva for bioethanol production. The engineered D. parva with higher starch and carbohydrate contents had a great potential for bioethanol production.