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<p>Lead (Pb) contamination poses a serious threat to soil ecosystems, crop productivity, and food safety. The use of Pb-resistant plant growth-promoting bacteria (PGPB) offers a sustainable and eco-friendly strategy for soil bioremediation. In this study, a novel Pb-tolerant PGPB strain, <i>Pseudomonas mendocina</i> L1, was isolated from phosphate rock-contaminated soil. Strain L1 exhibited high Pb resistance (MIC = 1000&#xa0;mg/L) and multiple plant growth-promoting traits, including phosphate solubilization, indole-3-acetic acid (IAA) and siderophore production, as well as ACC deaminase activity. Whole-genome sequencing (WGS) revealed that strain <i>P</i>. <i>mendocina</i> L1 harbors a 5.54&#xa0;Mb circular chromosome with a GC content of 62.39% and 5136 predicted coding sequences. Genomic analysis identified key genes involved in plant-beneficial functions, such as phosphate transport (<i>pstA</i>, <i>pstB</i>, <i>pstC</i>, <i>pstS</i>), siderophore-mediated iron uptake (<i>fhuB</i>, <i>fhuC</i>, <i>fhuD</i>), and IAA biosynthesis (<i>trpEDCBA</i> operon, <i>trpF</i>). Furthermore, a soil–pakchoi (<i>Brassica chinensis</i> L.) system was employed to evaluate its bioremediation potential, demonstrating that inoculation with <i>P</i>. <i>mendocina</i> L1 significantly improved plant growth while reducing Pb accumulation in edible tissues. These findings highlight <i>P</i>. <i>mendocina</i> L1 as a promising candidate for the bioremediation of Pb-contaminated soils, offering an eco-friendly and cost-effective strategy for sustainable agriculture and environmental restoration.</p> Graphical abstract <p></p>

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Integrated genomic and functional analysis of a Pb-resistant Pseudomonas mendocina L1 for phytoremediation of contaminated soils

  • 期刊论文

  • 发表日期：

    2025年12月2日

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  • Ying Jiao,
  • Guanqun Leng,
  • Maodi Ran,
  • Jiaxing Wu,
  • Yuxin Zhou,
  • Jiaokun Li

摘要

Lead (Pb) contamination poses a serious threat to soil ecosystems, crop productivity, and food safety. The use of Pb-resistant plant growth-promoting bacteria (PGPB) offers a sustainable and eco-friendly strategy for soil bioremediation. In this study, a novel Pb-tolerant PGPB strain, Pseudomonas mendocina L1, was isolated from phosphate rock-contaminated soil. Strain L1 exhibited high Pb resistance (MIC = 1000 mg/L) and multiple plant growth-promoting traits, including phosphate solubilization, indole-3-acetic acid (IAA) and siderophore production, as well as ACC deaminase activity. Whole-genome sequencing (WGS) revealed that strain P. mendocina L1 harbors a 5.54 Mb circular chromosome with a GC content of 62.39% and 5136 predicted coding sequences. Genomic analysis identified key genes involved in plant-beneficial functions, such as phosphate transport (pstA, pstB, pstC, pstS), siderophore-mediated iron uptake (fhuB, fhuC, fhuD), and IAA biosynthesis (trpEDCBA operon, trpF). Furthermore, a soil–pakchoi (Brassica chinensis L.) system was employed to evaluate its bioremediation potential, demonstrating that inoculation with P. mendocina L1 significantly improved plant growth while reducing Pb accumulation in edible tissues. These findings highlight P. mendocina L1 as a promising candidate for the bioremediation of Pb-contaminated soils, offering an eco-friendly and cost-effective strategy for sustainable agriculture and environmental restoration.

Graphical abstract