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<p>The present study proposes an integrated approach using a novel cyanobacterial-bacterial co-culture consisting of <i>Desertifilum tharense</i> MASKD1 and bacterial consortium DBPS1 to treat secondary sewage effluent in a raceway pond (50&#xa0;L). Noticeably, the synergistic growth was achieved in the undiluted secondary sewage effluent without supplementation of any nutrients. The cyanobacterial-bacterial co-culture system achieved an improved tertiary sewage treatment as compared to the use of individual cultures. The Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) analysis confirmed an efficient removal of multiple ECs. The 16S rRNA gene amplicon sequencing explained how the co-culture community dynamics might have contributed in the bioremediation process. The harvested biomass was further valorized for the recovery of phycocyanin by ammonium sulphate fractionation followed by size exclusion chromatography. The antioxidant activity of phycocyanin was evaluated by <i>Caenorhabditis elegans</i> lifespan assays. The median survival duration of <i>C. elegans</i> was 14 days, which increased 1.64 times (to 23 days) in the presence of phycocyanin. In addition, the catalase activity assay in <i>C. elegans</i> further supported this observation. The catalase activity decreased by 20 Units/mg in worms treated with H<sub>2</sub>O<sub>2</sub> along with phycocyanin compared to those treated with H<sub>2</sub>O<sub>2</sub> alone. This reduction might be attributed to the direct scavenging of ROS or the decreased intracellular accumulation of H<sub>2</sub>O<sub>2</sub> in the presence of phycocyanin. The studied cyanobacterium accumulated significant neutral lipid under nitrogen starvation, indicating its possible use as a biodiesel. Additionally, the findings supported the concept of a self-sustainable electrode design, wherein treated wastewater was innovatively utilized as the electrolyte in a Ni-fabric–based electrochemical cell. The green hydrogen and oxygen produced were comparable to the previous reports. Thus, this study demonstrates an interdisciplinary bio-electrochemical strategy by integrating wastewater remediation, bioproduct synthesis and renewable energy generation.</p> Graphical abstract <p></p>

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Synergistic co-culture of Desertifilum tharense MASKD1 and bacterial consortium DBPS1 in an integrated biorefinery for sewage remediation and recovery of phycocyanin, biodiesel and green hydrogen

  • 期刊论文

  • 发表日期：

    2025年12月5日

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  • Prasad Andhare,
  • Shrey Dube,
  • Bhargavi Sonavane,
  • Pratik M. Pataniya,
  • CK Sumesh,
  • Chirayu Desai,
  • Bhavtosh Kikani,
  • Datta Madamwar

摘要

The present study proposes an integrated approach using a novel cyanobacterial-bacterial co-culture consisting of Desertifilum tharense MASKD1 and bacterial consortium DBPS1 to treat secondary sewage effluent in a raceway pond (50 L). Noticeably, the synergistic growth was achieved in the undiluted secondary sewage effluent without supplementation of any nutrients. The cyanobacterial-bacterial co-culture system achieved an improved tertiary sewage treatment as compared to the use of individual cultures. The Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) analysis confirmed an efficient removal of multiple ECs. The 16S rRNA gene amplicon sequencing explained how the co-culture community dynamics might have contributed in the bioremediation process. The harvested biomass was further valorized for the recovery of phycocyanin by ammonium sulphate fractionation followed by size exclusion chromatography. The antioxidant activity of phycocyanin was evaluated by Caenorhabditis elegans lifespan assays. The median survival duration of C. elegans was 14 days, which increased 1.64 times (to 23 days) in the presence of phycocyanin. In addition, the catalase activity assay in C. elegans further supported this observation. The catalase activity decreased by 20 Units/mg in worms treated with H2O2 along with phycocyanin compared to those treated with H2O2 alone. This reduction might be attributed to the direct scavenging of ROS or the decreased intracellular accumulation of H2O2 in the presence of phycocyanin. The studied cyanobacterium accumulated significant neutral lipid under nitrogen starvation, indicating its possible use as a biodiesel. Additionally, the findings supported the concept of a self-sustainable electrode design, wherein treated wastewater was innovatively utilized as the electrolyte in a Ni-fabric–based electrochemical cell. The green hydrogen and oxygen produced were comparable to the previous reports. Thus, this study demonstrates an interdisciplinary bio-electrochemical strategy by integrating wastewater remediation, bioproduct synthesis and renewable energy generation.

Graphical abstract