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<p>The limited viability of plant growth-promoting bacteria (PGPB), particularly non-sporulating strains during storage and application, severely compromises biofertiliser efficacy. Nanofibre- based seed coatings represent a promising strategy to overcome this challenge. Herein, we developed a biohybrid coating by electrospinning an aqueous polyvinyl alcohol (PVA) solution containing either <i>Pseudomonas urmiensis</i> AW4 or <i>Bacillus pumilus</i> SF5 directly onto soybean seeds. Scanning electron microscopy (SEM) analysis revealed a homogeneous nanofibre coating, and energy- dispersive X- ray spectroscopy (EDS) confirmed its biohybrid nature. The final deposition of the material onto the seeds ranged from 3.0 to 3.7&#xa0;mg⋅ g<sup>− 1</sup>. Encapsulation within the nanofibres extended the viability of the non-sporulating AW4 strain to more than seven days, a significant improvement over the three days observed with standard inoculation. The spore-forming SF5 strain remained viable for over six months, with its integrity uncompromised by the high-voltage electrospinning process. Furthermore, the biohybrid coatings enhanced soybean germination parameters, including germination percentage, germination index, root length, and relative radical elongation. We propose that the hydrophilic nature of PVA and the high surface-area-to-volume ratio of the nanofibrous structure were key to this improved performance. This encapsulation approach is not only technically effective but also scalable, representing a promising platform to increase the field efficiency of microbial biofertilisers and promote sustainable agricultural systems.</p>

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From fibres to field: biohybrid coating of soybean (Glycine max. L) seeds with PGPB Pseudomonas urmiensis AW4 or Bacillus pumilus SF5 by electrospinning technology

  • 期刊论文

  • 发表日期：

    2025年11月28日

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  • Romina del Pilar Pramparo,
  • David Picón-Borregales,
  • Ana Laura Wevar Oller,
  • Paola Patricia Pereira,
  • Melina Andrea Talano,
  • Silvia Goyanes,
  • Elizabeth Agostini

摘要

The limited viability of plant growth-promoting bacteria (PGPB), particularly non-sporulating strains during storage and application, severely compromises biofertiliser efficacy. Nanofibre- based seed coatings represent a promising strategy to overcome this challenge. Herein, we developed a biohybrid coating by electrospinning an aqueous polyvinyl alcohol (PVA) solution containing either Pseudomonas urmiensis AW4 or Bacillus pumilus SF5 directly onto soybean seeds. Scanning electron microscopy (SEM) analysis revealed a homogeneous nanofibre coating, and energy- dispersive X- ray spectroscopy (EDS) confirmed its biohybrid nature. The final deposition of the material onto the seeds ranged from 3.0 to 3.7 mg⋅ g− 1. Encapsulation within the nanofibres extended the viability of the non-sporulating AW4 strain to more than seven days, a significant improvement over the three days observed with standard inoculation. The spore-forming SF5 strain remained viable for over six months, with its integrity uncompromised by the high-voltage electrospinning process. Furthermore, the biohybrid coatings enhanced soybean germination parameters, including germination percentage, germination index, root length, and relative radical elongation. We propose that the hydrophilic nature of PVA and the high surface-area-to-volume ratio of the nanofibrous structure were key to this improved performance. This encapsulation approach is not only technically effective but also scalable, representing a promising platform to increase the field efficiency of microbial biofertilisers and promote sustainable agricultural systems.