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<p>This study explores the extraction, optimization, and application of crude broth was used for soil/plant treatment, reserving purified EPS only for structural analysis produced by <i>Kocuria carniphila</i> AUMC B-453, isolated from Egyptian agricultural soil and identified via 16&#xa0;S rRNA sequencing. Characterization using Fourier Transform Infrared Spectroscopy (FTIR), Proton nuclear magnetic resonance (<sup>1</sup>HNMR), and High-Performance Liquid Chromatography (HPLC). confirmed the EPS as a heteropolysaccharide composed predominantly of arabinose, ribose, glucose, mannose, and xylose. Using the Plackett-Burman design, optimal EPS yield (13&#xa0;g/L) was achieved with 30&#xa0;g/L glycerol, 2&#xa0;g/L yeast extract, pH 7, and 48&#xa0;h incubation. Among substrates tested, glycerol and yeast extract were the most efficient carbon and nitrogen sources, producing 8.6&#xa0;g/L and 10&#xa0;g/L EPS, respectively. SEM analysis revealed a fibrous polymer matrix bridging sand particles, FTIR indicated strong chemical interactions between EPS and soil minerals. Under low irrigation, plants in EPS-treated soil showed increased growth: 17.7&#xa0;cm shoots and 23&#xa0;cm roots at 250 mL/2 kg soil. EPS treated soil demonstrated enhanced water retention, aggregation, and nano-porosity. These effects are attributed to dual physical and chemical mechanisms, contributing to reduced water loss and improved soil structure. The findings validate <i>K. carniphila</i> EPS as a potent, sustainable soil conditioner. This work supports microbial EPS as a promising tool for improving agricultural productivity in arid and sandy environments.</p>

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Improvement of water retention capacity in sandy soil with the application of a crude exopolysaccharide-containing fermentation broth produced by Kocuria carniphila AUMC B-453

  • 期刊论文

  • 发表日期：

    2025年11月13日

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  • Shimaa K. Ali,
  • Wael N. Hozzein,
  • Shereen M. Korany,
  • Mais Emad Ahmed,
  • Eman A. Hamed,
  • Hussein S. Mohamed

摘要

This study explores the extraction, optimization, and application of crude broth was used for soil/plant treatment, reserving purified EPS only for structural analysis produced by Kocuria carniphila AUMC B-453, isolated from Egyptian agricultural soil and identified via 16 S rRNA sequencing. Characterization using Fourier Transform Infrared Spectroscopy (FTIR), Proton nuclear magnetic resonance (1HNMR), and High-Performance Liquid Chromatography (HPLC). confirmed the EPS as a heteropolysaccharide composed predominantly of arabinose, ribose, glucose, mannose, and xylose. Using the Plackett-Burman design, optimal EPS yield (13 g/L) was achieved with 30 g/L glycerol, 2 g/L yeast extract, pH 7, and 48 h incubation. Among substrates tested, glycerol and yeast extract were the most efficient carbon and nitrogen sources, producing 8.6 g/L and 10 g/L EPS, respectively. SEM analysis revealed a fibrous polymer matrix bridging sand particles, FTIR indicated strong chemical interactions between EPS and soil minerals. Under low irrigation, plants in EPS-treated soil showed increased growth: 17.7 cm shoots and 23 cm roots at 250 mL/2 kg soil. EPS treated soil demonstrated enhanced water retention, aggregation, and nano-porosity. These effects are attributed to dual physical and chemical mechanisms, contributing to reduced water loss and improved soil structure. The findings validate K. carniphila EPS as a potent, sustainable soil conditioner. This work supports microbial EPS as a promising tool for improving agricultural productivity in arid and sandy environments.