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<p>We identified, isolated, and functionally characterized a cyclin-dependent kinase (CDK), PiPho85, from <i>Piriformospora indica.</i> The identified PiPho85 contains TY, PSTAIRE, protein kinase domain, and an ATP binding site which is highly conserved among the Pho85/CDK5 family protein specific for <i>Saccharomyces cerevisiae.</i> In a <i>S. cerevisiae</i> mutant strain (Δpho85), PiPho85 could regulate the acid phosphatase activity, restore the normal phenotype, confer salt- and drug-tolerance, and enable utilization of non-fermentable sugars. This suggests that PiPho85 complements the Pho85 function. The PiPho85 knock down (Kd) strain was generated by RNA interference (RNAi) and named as Kd-<i>PiPho85</i>-<i>P. indica</i>. The Kd strain was found susceptible to salt, drug and osmolarity stress and showed morphological abnormalities, abnormal-sized hyphae with large knot-like structure. This suggests that PiPho85 plays a regularity role in the physiology of <i>P. indica</i>. Further, Kd-<i>PiPho85</i> -<i>P. indica</i> colonized plants were found to be susceptible to salt stress and showed less growth than that of wild type (WT) <i>P. indica</i> colonized plants<i>.</i> This suggests that PiPho85 plays a crucial role in conferring resistance to WT <i>P. indica</i> colonized plants during salt stress.</p>

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PiPho85, a cyclin-dependent kinase of Piriformospora indica rescue colonized maize plants grown under salt stress

  • 期刊论文

  • 发表日期：

    2025年9月9日

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  • Arun Kumar Kashyap,
  • Paras Kumar,
  • Aayushi Bajaj,
  • Rupesh Aggarwal,
  • Pawan Kumar Sharma,
  • Pooja Panwar,
  • Shubhankar Pandey,
  • Vikas Yadav,
  • Ralf Oelmuller,
  • Meenakshi Dua,
  • Atul Kumar Johri

摘要

We identified, isolated, and functionally characterized a cyclin-dependent kinase (CDK), PiPho85, from Piriformospora indica. The identified PiPho85 contains TY, PSTAIRE, protein kinase domain, and an ATP binding site which is highly conserved among the Pho85/CDK5 family protein specific for Saccharomyces cerevisiae. In a S. cerevisiae mutant strain (Δpho85), PiPho85 could regulate the acid phosphatase activity, restore the normal phenotype, confer salt- and drug-tolerance, and enable utilization of non-fermentable sugars. This suggests that PiPho85 complements the Pho85 function. The PiPho85 knock down (Kd) strain was generated by RNA interference (RNAi) and named as Kd-PiPho85-P. indica. The Kd strain was found susceptible to salt, drug and osmolarity stress and showed morphological abnormalities, abnormal-sized hyphae with large knot-like structure. This suggests that PiPho85 plays a regularity role in the physiology of P. indica. Further, Kd-PiPho85 -P. indica colonized plants were found to be susceptible to salt stress and showed less growth than that of wild type (WT) P. indica colonized plants. This suggests that PiPho85 plays a crucial role in conferring resistance to WT P. indica colonized plants during salt stress.