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<p>Surfactants are amphiphilic molecules with a worldwide billionaire market. Although chemical surfactants are the most available and commonly used, the microbiologically produced congeners have attracted attention due to their low toxicity, biodegradability, and high ecological acceptability. Among microbial surfactants rhamnolipids stand out, with high yields of production, excellent surface-active properties and great potential market. However, the application of these compounds is still limited mostly because of excessive production costs. This study presents a cost-effective process for rhamnolipids production by semi-submerged static-cultivation using agar cubes as substrate. A concentration of 50&#xa0;g/L of rhamnolipids was reached in semi-submerged static-cultivation using agar cubes containing corn bran and soybean oil. Through this static process, besides avoiding foam, gradual release of nutrients provides a large increase in <i>P. aeruginosa</i> growth and consequently on rhamnolipids production.</p>

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Rhamnolipids production in semi-submerged static-cultivation using agar cubes as solid substrate

  • 期刊论文

  • 发表日期：

    2025年5月26日

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  • Mayara de Alencar Almeida,
  • Karen Stefany Conceição,
  • Juliana Perez Marques Teixeira,
  • Nicole Caldas Pan,
  • Luis Paulo Silveira Alves,
  • Gerson Nakazato,
  • Lucélia Donatti,
  • Emanuel M. Souza,
  • Doumit Camilios-Neto,
  • Josiane A. Vignoli

摘要

Surfactants are amphiphilic molecules with a worldwide billionaire market. Although chemical surfactants are the most available and commonly used, the microbiologically produced congeners have attracted attention due to their low toxicity, biodegradability, and high ecological acceptability. Among microbial surfactants rhamnolipids stand out, with high yields of production, excellent surface-active properties and great potential market. However, the application of these compounds is still limited mostly because of excessive production costs. This study presents a cost-effective process for rhamnolipids production by semi-submerged static-cultivation using agar cubes as substrate. A concentration of 50 g/L of rhamnolipids was reached in semi-submerged static-cultivation using agar cubes containing corn bran and soybean oil. Through this static process, besides avoiding foam, gradual release of nutrients provides a large increase in P. aeruginosa growth and consequently on rhamnolipids production.