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<p>The emerging contaminant 2, 6-di-tert-butyl-p-cresol (BHT) is a kind of synthetic phenolic antioxidant and can pose negative effects on the aquatic organism. However, the mechanism of phycosphere bacteria coordinating with microalgae in response to BHT stress remains poorly understood. Herein, the effect of BHT on the microalgae <i>Phaeodactylum tricornutum</i> was comprehensively analyzed. BHT exposure led to a dose-dependent inhibition of <i>P. tricornutum</i> growth and the photosynthetic pigment biosynthesis. BHT also led to an increase in the content of malondialdehyde, therefore microalgae responded to the oxidative stress by enhancing activities of antioxidant enzymes, including superoxide dismutase, catalase and peroxidase, to eliminate excess reactive oxygen species in the cells. Furthermore, transcriptome analysis revealed that genes related to photosynthesis, TCA cycle, oxidative phosphorylation, and indole-3-acetic acid (IAA) synthesis were up-regulated in response to BHT stress, which are crucial for the microalgae’s adaptation to stresses. In addition, high-throughput Illumina MiSeq sequencing results demonstrated a significant increase in the relative abundance of bacteria affiliated with <i>Halomonas</i>,<i> Marivita</i> and <i>Oceanicaulis</i>. Microbiological assays demonstrated that <i>Halomonas</i> can thrive by using BHT as the sole energy source and exhibit a chemotactic response to IAA. Therefore, we conclude that the increased content of IAA secreted by microalgae in the phycosphere environment promoted the enrichment of BHT-tolerant bacterium <i>Halomonas</i>, thereby it is helpful for environmental pressures adaptability of <i>P. tricornutum</i>. Overall, this study provided a comprehensive understanding of the physiological and biochemical effects of BHT on microalgae, and we highlight the potential functional significance of IAA in establishing an interaction between microalgae and algae-associated bacteria in adverse environments.</p>

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The influence of 2,6-Di-tert-butyl-p-cresol stress on the microalga Phaeodactylum tricornutum and phycosphere bacteria community

  • 期刊论文

  • 发表日期：

    2025年4月28日

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  • Xiao Lu,
  • Jie Liu,
  • Xinfeng Xiao,
  • Jianliang Xue,
  • Dongle Cheng,
  • Linlin Zhang

摘要

The emerging contaminant 2, 6-di-tert-butyl-p-cresol (BHT) is a kind of synthetic phenolic antioxidant and can pose negative effects on the aquatic organism. However, the mechanism of phycosphere bacteria coordinating with microalgae in response to BHT stress remains poorly understood. Herein, the effect of BHT on the microalgae Phaeodactylum tricornutum was comprehensively analyzed. BHT exposure led to a dose-dependent inhibition of P. tricornutum growth and the photosynthetic pigment biosynthesis. BHT also led to an increase in the content of malondialdehyde, therefore microalgae responded to the oxidative stress by enhancing activities of antioxidant enzymes, including superoxide dismutase, catalase and peroxidase, to eliminate excess reactive oxygen species in the cells. Furthermore, transcriptome analysis revealed that genes related to photosynthesis, TCA cycle, oxidative phosphorylation, and indole-3-acetic acid (IAA) synthesis were up-regulated in response to BHT stress, which are crucial for the microalgae’s adaptation to stresses. In addition, high-throughput Illumina MiSeq sequencing results demonstrated a significant increase in the relative abundance of bacteria affiliated with Halomonas, Marivita and Oceanicaulis. Microbiological assays demonstrated that Halomonas can thrive by using BHT as the sole energy source and exhibit a chemotactic response to IAA. Therefore, we conclude that the increased content of IAA secreted by microalgae in the phycosphere environment promoted the enrichment of BHT-tolerant bacterium Halomonas, thereby it is helpful for environmental pressures adaptability of P. tricornutum. Overall, this study provided a comprehensive understanding of the physiological and biochemical effects of BHT on microalgae, and we highlight the potential functional significance of IAA in establishing an interaction between microalgae and algae-associated bacteria in adverse environments.