Pre- and Post-Quercetin Effects Against Oxidative Stress Generated by Particulate Matter
摘要
Exposure to air pollution is closely linked to organic damage. Several studies have investigated increased disease incidence rates caused by air quality worsening, worldwide. Particulate matter (PM) is the major air pollution constituent linked to physiological disruptions. PM-related damage is associated with particles’ diameter and chemical composition, and it results in reactive oxygen species (ROS) formation. Quercetin is a polyphenolic flavonoid present in plants that has been tested as antioxidant compound to help minimizing oxidative stress-related damage. The current study treated rat cardiomyoblast cells (H9c2) with quercetin (50 µmol L−1), before and after their exposure to PM, and incubated them for 24 h. PM samples (PM10 and PM2.5) were herein used at 100% and 50% concentrations. The cell line H9c2 has been used as a model in PM exposures and also to verify the protective effects of antioxidants. Results have evidenced that both PM fractions enhanced ROS production and lipid peroxidation, as well as decreased antioxidant capacity. The PM10 (50%) group has shown increased cell viability and decreased lipoperoxidation, in both quercetin treatments. Cell viability in groups quercetin post-treated were equal to the ones observed for the controls in PM2.5 group (100%). The PM2.5 pre-exposure quercetin-treated group (50%) has shown increased total antioxidant capacity and decreased ROS compared to PM2.5 groups. In addition, PM10 (100% and 50%) presented significantly decreased ROS formation in the post-exposure quercetin treatment. These findings have indicated that quercetin can act as an important heart protector against oxidative stress associated PM effects in vitro pre- and post-treatment.